Recombinant organisms producing insect toxins and methods...

Details Recombinant organisms producing insect toxins and methods... Recombinant organisms producing insect toxins and methods...

2008-04-29

2008-04-29

Woitach, Joseph (Department: 1633)

Drug, bio-affecting and body treating compositions

Whole live micro-organism, cell, or virus containing

Genetically modified micro-organism, cell, or virus

C424S405000, C424S093461, C435S252100, C435S252300, C536S023710

Reexamination Certificate

active

11070575

ABSTRACT:
This invention is a method for constructing recombinant organisms that produce proteins lethal to the larvae of insects. Nucleotide sequences were isolated fromBacillus popilliaethat encode two adjacent, putative genes; orf1 and cryhime1. The cryhime1 sequence was related to otherBacillus popilliaegenes that encode proteins active against Scarabaeidae insect larvae. When these nucleotide sequences were transferred toBacillus thuringiensis, a protein was produced that had a lethal effect on the larvae from Scarabaeidae insects. When the orf1 sequence was removed from the recombinantBacillus thuringiensisstrain, no protein active against Scarabaeidae insect larvae was produced, strongly suggesting that the orf1 sequences are required for expression of the cryhime1 gene.

REFERENCES:
patent: 6204057 (2001-03-01), Schnetter et al.

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