Chemistry: molecular biology and microbiology – Vector – per se
Patent
1992-12-31
1997-11-25
Walsh, Stephen G.
Chemistry: molecular biology and microbiology
Vector, per se
4351723, 536 241, C12N 1563, C12N 1511
Patent
active
056911963
ABSTRACT:
The present invention describes a novel method for screening retinoid X receptor agonists or antagonists, comprising: using a retinoid X receptor expressed by a yeast expression system to screen a compound having a retinoid X receptor agonist or antagonist activity as well as a screen for detecting a compound having retinoid X receptor agonist or antagonist activity, which comprises the steps of (1) providing a yeast strain which expresses the retinoic acid receptor and activates a reporter plasmid containing apolipoprotein AI gene site A or a mutated variant thereof; (2) incubating the compound in suitable media and a colorless chromogenic substrate; and (3) examining the media for development of color.
REFERENCES:
Mol. Cel. Biol. 9(8):3517-3523, 1984, McDonnell et al. Reconstitution of the Vitamin D-Responsive Osteokalcin Transcription Unit in Saccharomyces cerevisiae.
Karathanasis. (1992). Apolipoprotein AI gene regulation . . . transcription factors. High Dens. Lipopro. & Atheroscl. III. pp. 21-31. (eds. Miller and Tall).
Klein-Hitpass et al. (1986). An estrogen responsive element . . . xenopus vitellogenin A2 gene functions in transfected human cells. Cell. 46:1053-1061.
Wilson et al. (1988). SRA5 encodes the Low-K.sub.m cyclic AMP phosphodiesterase of Saccharomyces cerevisiae. Mol. Cell Biol. 8(1): 505-510.
Beato, M. (1989). Gene regulation by steroid hormones. Cell. 56:335-344.
Rottman et al. (1991). A retinoic acid-responsive element in the apolipoprotein AI gene . . . retinoic acid response pathways. Mol. Cell Biol. 11(7):3814-3820.
O'Malley, B. (1990). The steroid receptor superfamily: more excitement predicted for the future. Mol. Endocrin. 4(3):363-369.
Hermann et al. (1992). Heterodimeric receptor complexes determine 3,5,3'-triiodothyronine and retinoid signaling specificitie. Mol. Endocrin. 6(7):1153-1162.
de The et al. (1990). Identification of a retinoic acid responsive element in the retinoic acid receptor .beta. gene. Nature. 343:177-180.
Zhang et al. (1992). Retinoid X receptor is an auxillary protein for thyroid hormone and retinoic acid receptors. Nature. 355:441-445.
Kliewer et al. (1992). Retinoid X receptor interacts with nuclear receptors in retinoic acid, thyroid hormone and vitamin D.sub.3 signalling. Nature. 355:446-449.
Levin et al. (1992). 9-Cis retinoic acid stereoisomer binds and activates the nuclear receptor RXR.alpha.. Nature. 355:359-361.
Mangelsdorf et al. (1990). Nuclear receptor that identifies a novel retinoic acid response pathway. Nature. 345:224-229.
Mak et al. (1989). Expression of functional chicken oviduct progesterone receptors in yeast. J. Biol. Chem. 264(36):21613-21618.
Leroy et al. (1991). Mouse retinoic acid receptor .alpha.2 isoform is transcribed . . . retinoic acid response element. Proc. Natl. Acad. Sci. USA. 88:10138-10142.
Bugge et al. (1992). RXR.alpha., a promiscuous partner of retinoic acid and thyroid hormone receptors. EMBO J. 11(4):1409-1418.
Ladias et al. (1991). Regulation of the Apolipoprotein AI Gene by ARP-1, a novel member of the steroid receptor superfamily. Science. 251:561-565.
Heyman et al. (1992). 9-Cis retinoic acid is a high affinity ligand for the retinoid x receptor. Cell. 68:397-406.
Widom et al. (1991). Synergistic interactions between transcription factors control expression of the apolipoprotein AI gene in liver cells. Mol. Cell Biol. 11(2):677-687.
Karathanasis Sotirios K.
Mak Paul
American Cyanamid Company
Rosenblum Anne M.
Ulm John D.
Walsh Stephen G.
Webster Darryl L.
LandOfFree
Recombinant nucleic acid containing a response element does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Recombinant nucleic acid containing a response element, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Recombinant nucleic acid containing a response element will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2106095