Recombinant negative strand RNA virus expression systems and vac

Details Recombinant negative strand RNA virus expression systems and vac Recombinant negative strand RNA virus expression systems and vac

1994-10-14

1998-07-28

Degen, Nancy

Chemistry: molecular biology and microbiology

Virus or bacteriophage, except for viral vector or...

Recovery or purification

4351723, 435194, 4352351, 4353201, 536 231, 536 2372, C12N 702, C12N 701, C12N 912, C12N 1544

Patent

active

057861994

ABSTRACT:
Recombinant negative-strand viral RNA templates are described which may be used with purified RNA-directed RNA polymerase complex to express heterologous gene products in appropriate host cells and/or to rescue the heterologous gene in virus particles. Heterologous gene products include peptides or proteins derived from HIV which may be presented by a chimeric influenza virus to generate an immune response that is protective against challenge with HIV. A chimeric virus is described which contains an HIV peptide inserted into an influenza protein and which induced both humoral and cell-mediated immune responses against HIV. The RNA templates are prepared by transcription of appropriate DNA sequences with a DNA-directed RNA polymerase. The resulting RNA templates are of the negative-polarity and contain appropriate terminal sequences which enable the viral RNA-synthesizing apparatus to recognize the template.

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