Recombinant negative strand RNA virus

Details Recombinant negative strand RNA virus Recombinant negative strand RNA virus

1994-03-10

1996-11-26

Mosher, Mary E.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

4352351, 4353201, 435236, 935 32, C12N 1509, C12N 701, C12N 702, C12N 704

Patent

active

055784739

ABSTRACT:
Recombinant negative strand virus RNA templates which may be used to express heterologous gene products and/or to construct chimeric viruses are described. Influenza viral polymerase, which was prepared depleted of viral RNA, was used to copy small RNA templates prepared from plasmid-encoded sequences. Template constructions containing only the 3' end of genomic RNA were shown to be efficiently copied, indicative that the promoter lay solely within the 15 nucleotide 3' terminus. Sequences not specific for the influenza vital termini were not copied, and, surprisingly, RNAs containing termini identical to those from plus sense cRNA were copied at low levels. The specificity for recognition of the virus-sense promoter was further defined by site-specific mutagenesis. It was also found that increased levels of vital protein were required in order to catalyze both the cap-endonuclease primed and primer-free RNA synthesis from these model templates as well as from genomic length RNAs. This indicated that this reconstituted system had catalytic properties very smilar to those of native viral RNPs. High levels of expression of a heterologous gene was obtained using the constructs and methods described.

REFERENCES:
patent: 4786600 (1988-11-01), Kramer et al.
patent: 5166057 (1992-11-01), Palese et al.
Tyler, K. L. et al. 1991, Fundamental Virology, 2nd Ed., B. N. Fields et al, Raven Press, N.Y., pp. 191-239.
Westrop, G. D. et al. 1987, In The Molecular Biology of the Positive Strand RNA Viruses, Academic Press, London, pp. 53-60.
Lustig, S. et al., 1988, J. Virol. vol. 62 pp. 2329-2336.
Kinney, R. M. et al. 1989, Virology vol. 170 pp. 19-30.
Emerson and Yu, 1975, "Both NS and L Proteins Are Required for In Vitro RNA Synthesis by Vesicular Stomatitis Virus", J. Virol. 15: 1348-1356.
Naito and Ishihama, 1976, "Function and Structure of RNA Polymerase from Vesicular Stomatitis Virus", J. Biol. Chem. 251: 4307-4314.
Hay et al., 1977, "Transcription of the Influenza Virus Genome", Virol. 83: 337-355.
Racaniello et al., 1981, "Cloned Poliovirus Complementary DNA Is Infectious in Mammalian Cells", Science 214: 916-919.
Lamb and Choppin, 1983, "The Gene Structure and Replication of Influenza Virus", Ann. Rev. Biochem 52: 467-506.
Krug, Transcription and Replication of Influenza Viruses. In: Genetics of Influenza Viruses, Ed., Palese, P. and Kingsbury, D. W. New York, Springer-Verlag, 1983, pp. 70-98.
Dreher et al., 1984, "Mutant Viral RNAs Synthesized In Vitro Show Altered Aminoacylation and Replicase Template Activities", Nature 311: 171-175.
Dreher et al., 1988, "Mutational Analysis of the Sequence and Structural Requirements in Brome Mosaic Virus RNA for Minus Strand Promoter Activity", J. Mol. Biol. 201: 31-40.
Kaplan et al., 1985, "In Vitro Synthesis of Infectious Poliovirus RNA", Proc. Natl. Acad. Sci. USA. 82: 8424-8428.
Kato et al., 1985, "Purification and Enzymatic Properties of an RNA Polymerase-RNA Complex from Influenza Virus", Virus Research 3: 115-127.
De and Banerjee, 1985, "Requirements and Functions of Vesicular Stomatitis Virus L and NS Proteins in the Transcription Process In Vitro", Biochem. Biophys. Res. Commun. 126: 40-49.
Beaton and Krug, 1986, "Transcription Antitermination During Influenza Viral Template RNA Synthesis Requires the Nucleocapsid Protein and the Absence of a 5' Capped End", Proc. Natl. Acad. Sci. USA. 83: 6282-6286.
Levis et al., 1986, "Deletion Mapping of Sindbis Virus DI RNAs Derived from a cDNAs Defines the Sequences Essential for Replication and Packaging", Cell 44: 137-145.
Takeuchi et al., 1987, "In Vitro Synthesis of Influenza Viral RNA: Characterization of an Isolated Nuclear System That Supports Transcription of Influenza Viral RNA", J. Biochem. 101:837-845.
Hsu et al., 1987, "Genomic RNAs of Influenza Viruses Are Held in a Circular Conformation In Virions and In Infected Cells by a Terminal Panhandle", Proc. Natl. Acad. Sci. USA. 84: 8140-8144.
Honda et al., "Identification of the RNA Polymerase-Binding Site on Genome RNA of Influenza Virus", J. Biochem. 102: 1241-1249.
Ward et al., 1988, "Direct Measurement of the Poliovirus RNA Polymerase Error Frequency In Vitro", J. Virol. 62: 558-562.
Mirakhur and Peluso, 1988, "In Vitro Assembly of a Functional Nucleocapsid from the Negative-Stranded Genome RNA of a Defective Interfering Particle of Vesicular Stomatitis Virus", Proc. Natl. Acad. Sci. USA. 85: 7511-7515.
Ishihama and Nagata, 1988, "Viral RNA Polymerases", CRC Crlt. Rev. Biochem. 23: 27-76.
Shapiro and Krug, 1988, "Influenza Virus RNA Replication In Vitro: Synthesis of Viral Template RNAs and Virion RNAs in the Absence of an Added Primer", J. Virol. 62: 2285-2290.
Honda et al., 1988, "RNA Polymerase of Influenza Virus: Role of NP in RNA Chain Elongation", J. Biochem. 104: 1021-1026.
Szewczyk et al., 1988, "Modification, Thioredoxin Renaturation, and Reconstituted Activity of the Three Subunits of the Influenza A Virus RNA Polymerase", Proc. Natl. Acad. Sci. USA 85: 7907-7911.
Palese, 1977, "The Genes of Influenza Virus", Cell, 10: 1-10.
Bishop et al., 1971, "Transcription of the Influenza Ribonucleic Acid Genome by a Virion Polymerase", J. Virol. 8: 66-73.
Bouloy et al., 1980, "Both the 7-methyl and the 2'-O-methyl Groups in the Cap of mRNA Strongly Influence its Ability to Act as Primer for Influenza Virus RNA Transcription", Proc. Natl. Acad. Sci. USA. 77: 3952-3956.
Ulmanen et al., 1983, "Influenza Virus Temperature-Sensitive Cap (m.sup.7 GpppNm)-Dependent Endonuclease", J. Virol. 45: 27-35.
Beaton and Krug, 1984, "Synthesis of the Templates for Influenza Virion RNA Replication In Vitro", Proc. Natl. Acad. Sci. USA. 81: 4682-4686.
Kawakami et al., 1981, "RNA Polymerase of Influenza Virus. II. Influence of Oligonucleotide Chain Length on the Priming Activity of RNA Synthesis", J. Biochem. 89: 1759-1768.
Kawakami and Ishihama, 1983, "RNA Polymerase of Influenza Virus. III. Isolation of RNA Polymerase-RNA Complexes from Influenza Virus PR8", J. Biochem. 93: 989-996.
Detjen et al., 1987, "The Three Influenza Virus Polymerase (P) Proteins Not Associated with Viral Nucleocapsids in the Infected Cell Are in the Form of a Complex", J. Virol. 61: 16-22.
St. Angelo et al., 1987, "Two of the Three Influenza Viral Polymerase Proteins Expressed by Using Baculovirus Vectors Form a Complex in Insect Cells", J. Virol. 61: 361-365.
Khan et al., 1987, "Synthetic Templates and the RNA Polymerase of Influenza A Virus", Nucleosides & Nucleosides 6: 543-554.
Krystal et al., 1986, "Expression of the Three Influenza Virus Polymerase Proteins in a Single Cell Allows Growth Complementation of Viral Mutants", Proc. Natl. Acad. Sci. USA. 83: 2709-2713.
Li et al., 1989, "Complementation and Analysis of an NP Mutant of Influenza Virus", Virus Research, 12: 97-112.
Kingsbury, et al., 1987, "Assembly of Influenza Ribonucleoprotein In Vitro Using Recombinant Nucleoprotein", Virol. 156: 396-403.
Rochovansky, 1976, "RNA Synthesis by Ribonucleoprotein-Polymerase Complexes Isolated from Influenza Virus", Virol. 73: 327-338.
Robertson et al., 1981, "Polyadenylation Sites for Influenza Virus mRNA", J. Virol. 38: 157-163.
Schreirer et al., 1988, "Functional and Structural Analysis of the Ribonucleoprotein Complexes of Different Human Influenza Virus Strains", Acta. Virol. 32: 289-295.
Xiong et al., 1989, "Sindbis Virus: An Efficient, Broad Host Range Vector for Gene Expression in Animal Cells", Science, 243: 1188-1191.
Parvin et al., 1989, "Promoter Analysis of Influenza Virus RNA Polymerase", J. Virol. 63: 5142-5152.
Luytjes et al., 1989, "Amplification, Expression, and Packaging of a Foreign Gene by Influenza Virus", Cell 59: 1107-1113.
Enami et al., 1990, "Introduction of Site-Specific Mutations Into The Genome of Influenza Virus", Proc. Natl. Acad. Sci. 87: 3802-3805.
Ballart et al., 1990, "Infectious Measles Virus from Cloned cDNA", EMBO J. 9: 379-384; and its retraction at 8th International Conference on Negative Strand Viruses, 1991, Abstr. 43.
Huang et al., 1990, "Determination of Influenza Virus Proteins Required for Genome Replication", J. Virol. 64: 5669-5673.
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