Recombinant narbonolide polyketide synthase

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

435132, 4352523, 43525233, 43525235, 4353201, 435189, 536 232, 536 237, C12P 702

Patent

active

061176591

ABSTRACT:
Recombinant DNA compounds that encode all or a portion of the narbonolide polyketide synthase are used to express recombinant polyketide synthase genes in host cells for the production of narbonolide, narbonolide derivatives, and polyketides that are useful as antibiotics and as intermediates in the synthesis of compounds with pharmaceutical value.

REFERENCES:
patent: 4551433 (1985-11-01), DeBoer
patent: 4874748 (1989-10-01), Katz et al.
patent: 5063155 (1991-11-01), Cox et al.
patent: 5098837 (1992-03-01), Beckmann et al.
patent: 5149639 (1992-09-01), Katz et al.
patent: 5168052 (1992-12-01), Cox et al.
patent: 5252474 (1993-10-01), Gewain et al.
patent: 5514544 (1996-05-01), Rao et al.
patent: 5672491 (1997-09-01), Khosla et al.
patent: 5712146 (1998-01-01), Khosla et al.
patent: 5712496 (1998-01-01), Takahashi et al.
patent: 5824513 (1998-10-01), Katz et al.
Bartel, et al., "Biosynthesis of anthraquinones by interspecies cloning of actinorhodin biosynthesis genes in streptomycetes: Clarification of actinorhodin gene functions," J Bacteriol (1990) 172(9):4816-4826.
Beck, J., et al., "the multifunctional 6-methylsalicylic acid synthase gene of Penicillium patulum its gene structure relative ot that of other polyketide synthases," Eur J Biochem (1990) 192:487-498.
Bibb, et al., "Analysis of the nucleotide sequence of the Streptomyces glaucescens tcml genes provides key information about the enzymology of polyketide antibiotic biosynthesis," EMBO J (1989) 8(9):2727-2735.
Brown, M.J.B. et al., "A Mutant Generated by Expression of an Engineered DEBS1 Protein from the Erythromycin-Producing Polyketide Synthase (PKS) in Streptomyces Coelicolor Produces the Triketide as a Lactone, but the Major Product is the Nor-Analogue Derived from Acetate as Starter Acid," Journal of the Chemical Society, Chemical Communications, GB, Chemical Society. No. 15, 1995, pp. 1517-1518, XP002044729 ISSN: 0022-4936.
Caballero et al., "Organisation and functions of the actVA region of the actinorhodin biosynthetic gene cluster of Streptomyces coelicolor," Mol Gen Genet (1991) 230:401-412.
Caffrey et al., FEBS Lett. (1992), 304:225-228.
Cane, D.E. et al., J. Am. Chem. Soc. (1993), 115:522-526.
Cane, D.E. et al., J. Antibiotics (1995), 48:647-651.
Cortes, J., et al., "An unusually large multifunctional polypeptide in the erythromycin-producing polyketide synthase of Saccharopolyspora erythraea," Nature (Nov. 8, 1990) 348:176-178.
Dalbie-McFarland et al., Proc Natl Acad Sci USA (1982), 79:6409.
Donadio et al., "Biosynthesis of the erythromycin macrolactone and a rational approach for producing hybrid macrolides," Gene (1992) 115:97-103.
Donadio et al., Industrial Microorganism, Basic and Applied Molecular Genetics 1993, R.H. Baltz, G.D. Hegeman and PIL. Skatrud (eds) (Amer. Soc. Microbial), Washington, D.C. p. 257-265.
Donadio, S. et al., Proc Natl Acad Sci USA (1993), 90:7119-7123.
Donadio, S., et al., "Modular Organization of Genes Required for Complex Polyketide Biosynthesis," Science (May 3, 1991) 252:675-679.
Evans, D.A. et al., J. Am. Chem. Soc. (1992), 114:9434-9453.
Fernandez-Moreno et al., "Nucleotide sequence and deduced functions of a set of cotranscribed genes of Streptomyces coelicolor A3(2) including the polyketide synthase for the antibiotic actinorhodin," J Biol Chem (1992) 267:19278-19290.
Fernandez-Moreno et al., "the act cluster contains regulatory and antibiotic export genes, direct targets for translational control by the bldA tRNA gene of Streptomyces," Cell (1991) 66:769-780.
Floss, "Genetic engineering of hybrid antibiotics--a progress report," Tetrahydron (1991) 47(31):6045-6058.
Fraley, R.T., et al., "Expression of bacterial genes in plant cells," Proc Natl Acad Sci USA (1983), 80:4803-4807.
Fu, "Engineered biosynthesis of novel polyketides: Stereochemical course of two reactions catalyzed by a polyketide synthase," Biochemistry (1994) 33(31):9321-9326.
Geisselsoder, J., et al., "Efficient site-directed in vitro mutagenesis," BioTechniques (1987), 5:786-791.
Hallam, "Nucleotide sequence, transcription and deduced function of a gene involved in polyketide antibiotic synthesis in Streptomyces coelicolor," Gene (1988) 74:305-320.
Hamilton et al., J. Bacteriol (1989), 171:4617.
Hopwood et al., "Product of `hybrid` antibiotics by genetic engineering," Nature (1985) 314 (6012):642-644.
Ireland, R.E., et al., J. Org. Chem, (1980), 45:1868-1880.
Jay, E. et al., J. Biol. Chem. (1984), 259:6311-6317.
Kao, C.M. et al., J. Am. Chem. Soc. (1994), 116:11612-11613.
Kao, C.M. et al., Science (1994), 265:509-512.
Katz et al., "Polyketide synthesis Prospects for hybrid antibiotics," Ann. Review Miocrobiol (1993) 47:875-912.
Khosla, Chaitan et al., "Generation of polyketide libraries via combinatorial biosynthesis," Tibtech Sep. 1996 (vol. 14) pp. 335-341.
Khosla, C., et al., "Genetic construction and functional analysis of hybrid polyketide synthases containing heterologous acyl carrier proteins," J Bacteriol (1993), 175:2197-2204.
Khosla, et al., "Targeted gene replacements in a Streptomyces polyketide synthase gene cluster: role for the acyl carrier protein," Mole Microbiol (1992) 6(21):3237-3249.
Kuhstoss, S. et al., Gene (1996) 183:231-236.
Kunkel, T.A., Proc Natl Acad Sci USA (1985), 82:488.
Lambalot, R.H. et al., J. Antibiotics (1992), 45:1981-1982.
Lehrer, R. et al., J. Immunol Meth (1991), 137:167-173.
MacNeil, D.J., J. Bacteriol (1988), 170:5607.
MacNeil, D.J., et al., "Complex organization of the Streptomyces avermitilis genes encoding the avermectin polyketide synthase," Gene (1992) 115:119-125.
Malpartida et al., "Homology between Streptomyces genes coding for synthesis of different polyketides used to clone antibiotic biosynthetic genes," Nature (1987) 325(6107):818-821.
Malpartida et al., "Physical and genetic characterisation of the gene cluster for the antibiotic actinorhodin in Streptomyces coelicolor A3(2)," Mol Gen Genet (1986) 205:66-73.
Malpartida, F., et al., "Molecular cloning of the whole biosynthetic pathway of a streptomyces antibiotic and its expression in a heterologous host," Nature (1984), 309:462-464.
Marsden, A.F.A., et al., "Engineering Broader Specificity into an Antibiotic-Producing Polyketide Synthase," Science (Jan. 9, 1998) 279:199-202.
McDaniel et al., 1993 "Engineered biosynthesis of novel polyketides", Science 262:1546-1550 (1993).
Martin, S.F. et al., J. Am. Chem. Soc. (1997), 119:3193.
Masamune et al., J. Am. Chem. Soc. (1975), 97:3512-3513.
Masumoto, T. et al., Tetrohedron Lett.(1988), 29:3575.
Betlach, M.C., et al., "Characterization of the Macrolide P-450 Hydroxylase from Streptomyces venezuelae which Converts Narbomycin t Picromycin," Bichemistry (1998) 37:14937-14942.
Oliynyk, M., et al., "A hybrid modular polyketide synthase obtained by domain swapping," Chemistry & Biology (Oct. 1996) 3:833-839.
Perun, T.J., Drug Action and Drug Resistance in Bacteria, vol. 1, S. Mitsuhashi (ed) Univ. Park Press, Baltimore, 1977.
Sherman et al., "Functional replacement of genes for individual polyketide synthase components in Streptomyces coelicolor A3(2) by heterogenous genes from a different polyketide pathway," J Bacteriol (1992) 174:6184-6190.
Sherman et al., "Structure and deduced function of the granaticin-producing polyketide synthase gene cluster of Streptomyces violaceroruber Tu22," EMBO J (1989) 8:2717-2725.
Toshima, K. et al., J. Am. Chem. Soc. (1995), 117:3717.
Tuan et al., Gene (1990), 90:21-29.
Vedejs, E. et al., J Am Chem Soc (1987), 109:5437-5446.
Vedejs, E. et al., J Am Chem Soc (1989), 111:8430-8438.
Weber, J.M. et al, "Genetic Analysis of Erythromycin Production in Streptomyces erythreus," J. of Bacteriology, vol. 164, No. 1, issued Oct. 1985, pp. 425-433, See the entire document.
Weber, J.M. et al, "Organization of a Cluster of Erythromycin Genes in Saccharomyces erythraea," J. of Bacteriology, vol. 172, No. 5, issued May 1990, pp. 2372-2383. See the entire document.
Woodward, R.B. et al., J. Am. Chem. Soc. (1981), 103:3215.
Zoller, et al., Methods in Enzymology (1983), 100:468.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Recombinant narbonolide polyketide synthase does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Recombinant narbonolide polyketide synthase, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Recombinant narbonolide polyketide synthase will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-94938

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.