Recombinant mycobacteria overexpressing D-alanine ligase...

Chemistry: molecular biology and microbiology – Vector – per se

Reexamination Certificate

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C435S252300, C435S253100, C435S069100, C435S183000, C435S004000, C530S350000, C536S023200

Reexamination Certificate

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07371571

ABSTRACT:
Recombinant mycobacterial strains which overproduce essential biosynthetic enzymes of pathogenic mycobateria are provided. These strains overproduce enzymes involved in the synthesis and incorporation ofD-alanine into mycobacterial peptidoglycan, the backbone of the mycobacterial cell wall. These overproducing strains may be used as reference strains in in vitro screening methods to identify antimycobacterial agents.

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