Recombinant methods of making gamma interferon compositions

Details Recombinant methods of making gamma interferon compositions Recombinant methods of making gamma interferon compositions

1994-03-24

1997-01-21

Fitzgerald, David L.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

530351, 424 855, C12N 1523, C12N 121

Patent

active

055958885

ABSTRACT:
Provided are methods for the production of mixtures of interferon (IFN)-.gamma. polypeptides. The methods employ a single species of IFN-.sub..gamma. DNA having codons for Met-Gln and for residues 2-143 of the mature human IFN-.sub..gamma. amino acid sequence. Expression of such DNA in a suitable recombinant host cell affords a mixture of polypeptides having N-terminal Met-Gln, H-Gln, or H-pyroglutamate residues and C-termini at Arg.sup.139, Ser.sup.142, or Gln.sup.143 of the mature IFN-.sub..gamma. sequence. The polypeptides so produced exhibit the antiviral and antiproliferative activities as well as the acid lability characteristic of native human IFN-.sub..gamma..

REFERENCES:
patent: 4285929 (1981-08-01), Sugimoto et al.
patent: 4332892 (1982-06-01), Ptashne et al.
patent: 4338397 (1982-07-01), Gilbert et al.
patent: 4382027 (1983-05-01), Braude
patent: 4457867 (1984-07-01), Ishida et al.
patent: 4599306 (1986-07-01), Altrock
patent: 4604284 (1986-08-01), Kung et al.
patent: 4727138 (1988-02-01), Goeddel et al.
patent: 4762791 (1988-08-01), Goeddel et al.
patent: 4855238 (1989-08-01), Gray et al.
patent: 4855409 (1989-08-01), Masakazu et al.
patent: 4925793 (1990-05-01), Goeddel et al.
patent: 4929554 (1990-05-01), Goeddel et al.
patent: 5004689 (1991-04-01), Fiers et al.
Novokhatskii et al, Chemical Abstract 96, No. 120770e (1982) first pub. in Dokl. Akad. Nauk. SSSR 261, 997 (1981).
Goeddel et al, Nature 287, 411 (1980).
Hamer et al, Nature 281, 35 (1979).
Weissman, in Interferon 3, (Gresser, Ed: Academic Press) 101 (1981).
Vilcek, in Interferon 4, (Academic Press) 129 (1982).
Murray, Annals of Internal Medicine 98, 1016 (1983).
Maniatis et al, Molecular Cloning, A Laboratory Manual, 1982, Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y., pp. 418-419.
Devos et al, "Molecular Cloning of Human Immune Interferon cDNA and its expression in Eukaryotic cells", Nucleic Acids, Res. 10:2487 (1982).
Fiers et al, "Molecular-biological studies on human fibroblast interferon, immune interferon and Interleukin 2 Genes", in The Biology of the Interferon System 1983, DeMaeyer et al (ed.), 1983, Elsevier Science Publishers, Amsterdam, pp. 3-8.
Alton et al, "Production, Characterization and biological effects of Recombinant DNA Derived Human IFN-alpha and IFN-gamma analogs", in The Biology of the Interferon System 1983, DeMaeyer et al (ed.), 1983, Elsevier Science Publishers, Amsterdam, pp. 119-128.
Hsieh et al, J. Biol. Chem., vol. 258, pp. 2548-2554, (1983).
Yamashita et al., Archives of Biochemistry, vol. 225, pp. 993-996 (1983).
Nakamura et al., Virology, vol. 95, pp. 8-23, (1979).
Concanavalin A, Pharmacia Fine Chemicals.
Gene Expression, vol. 2, Eucaryotic Chromosomes, Lewin 1974, John Wiley & Sons, New York, p. 148.
Grey, et al, "Expression of Human Immune Interferon cDNA in E. Coli and Monkey Cells", Nature 295; 503 (1982).
Yip et al, Science 215, 411 (1982).
Genetic Engineering Letter 2, No. 17 "Artificial Gene for Gamma Interferon Reported by Japanese Scientist" (1982).
Newmark, Nature 294, 7 (1981).
Luskey et al, Nature 293, 79 (1981).
Research Disclosure, "Purification of Interferon mRNA by Hybridising Induced Material to cDNA", (10 July 1979).
O'Malley, Methods in Enzymology, vol. 78, pp. 540-545, (1981).
Life, p. 50, (May 1980).
Time, p. 60, (Mar. 31, 1980).
Devos, et al, Nucl. Acids. Res. 10, 2487 (1982).
Epstein, Nature 295, 453 (1982).
Epstein, in Interferon 3, (Gresser, ed: Academic Press) 13 (1981).
Nathan et al, Nature 292, 842 (1981).
Dianzani et al, Nature 283, 400 (1980).
Wallace et al, Fed. Proc. 40, 1574 (1981).
Wallace et al, Biochem. Biophys. Res. Comm. 100, 865 (1981).
Taniguchi et al, Proc. Natl. Acad. Sci. USA 78, 3469 (1981).
Epstein, L. B., Fed. Proc. 40, 56 (1981).
Vilcek, J. et al, Microbiol., 204 (1980).
Capon et al, The Third Annual International Congress for Interferon Research, Miami, Florida, 1982, p. 15.
Derynck et al, Experimental Manipulation of Gene Expression, p. 247 (1983).
de Ley, M. et al; Euro. J. Immunol. 10, 877 (1980).
Yip, Y. K. et al; Proc. Nat. Acad. Sci. (USA), 78, 1601 (1978).
Berger, S. L. et al, J. Biol. Chem. 255, 2955 (1980).
Botstein et al, Recomb. DNA Tech. Bull. 2, 49 (1979).

Affiliated with

Also associated with

Rating

Recombinant methods of making gamma interferon compositions does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Recombinant methods of making gamma interferon compositions, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Recombinant methods of making gamma interferon compositions will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2323467