Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Reexamination Certificate
2003-12-03
2008-03-18
Landsman, Robert S. (Department: 1647)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
C435S252300, C435S471000
Reexamination Certificate
active
07344859
ABSTRACT:
Methods for expressing a functional heteromeric taste receptor that responds to sweet taste stimuli are provided. These methods comprise the co-expression of T1R2 and T1R3 nucleic acid sequences in a host cell that desirably further expresses a G protein that couples therewith, e.g., Gα15, Gα16or gustducin. In preferred embodiments, the host cells will be mammalian cells or Xenopus oocytes. These nucleic acid sequences are expressed constitutively or under inducible conditions. In preferred embodiments the expression methods will use HEK-293 cells that also stably express Gα15. These methods give rise to heteromeric receptors and compositions containing that are useful in assays for identifying novel sweeteners and sweetness modulators.
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Adler Jon Elliot
Echeverri Fernando
Li Xiaodong
O'Connell Shawn
Staszewski Lena
Hunton & Williams LLP
Landsman Robert S.
Senomyx Inc.
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