Recombinant methods and materials for producing epothilone...

Details Recombinant methods and materials for producing epothilone... Recombinant methods and materials for producing epothilone...

2005-02-22

2005-02-22

Nashed, Nashaat T. (Department: 1652)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

C435S183000, C435S252310, C435S252330, C536S023100, C536S023200, C536S023700

Reexamination Certificate

active

06858411

ABSTRACT:
Recombinant nucleic acids that encode all or a portion of the epothilone polyketide synthase (PKS) are used to express recombinant PKS genes in host cells for the production of epothilones, epothilone derivatives, and polyketides that are useful as cancer chemotherapeutics, fungicides, and immunosuppressants.

REFERENCES:
patent: 4916138 (1990-04-01), Ueda et al.
patent: 5605793 (1997-02-01), Stemmer
patent: 5672491 (1997-09-01), Khosla et al.
patent: 5686295 (1997-11-01), Jaoua et al.
patent: 5712146 (1998-01-01), Khosla et al.
patent: 5776735 (1998-07-01), Denoya et al.
patent: 5783431 (1998-07-01), Peterson et al.
patent: 5811238 (1998-09-01), Stemmer et al.
patent: 5824513 (1998-10-01), Katz et al.
patent: 5830721 (1998-11-01), Stemmer et al.
patent: 5830750 (1998-11-01), Khosla et al.
patent: 5843718 (1998-12-01), Khosla et al.
patent: 5969145 (1999-10-01), Schinzer et al.
patent: 6022731 (2000-02-01), Khosla et al.
patent: 6033883 (2000-03-01), Barr et al.
patent: 6090601 (2000-07-01), Gustafsson et al.
patent: 6121029 (2000-09-01), Schupp et al.
patent: 6242469 (2001-06-01), Danishefsky et al.
patent: 6300355 (2001-10-01), Danishefsky et al.
patent: 6303342 (2001-10-01), Julien et al.
patent: 6346404 (2002-02-01), Schupp et al.
patent: 6355457 (2002-03-01), Schupp et al.
patent: 6355458 (2002-03-01), Schupp et al.
patent: 6355459 (2002-03-01), Schupp et al.
patent: 6358719 (2002-03-01), Schupp et al.
patent: 6383787 (2002-05-01), Schupp et al.
patent: 6391594 (2002-05-01), Khosla et al.
patent: 6410301 (2002-06-01), Julien et al.
patent: 6489314 (2002-12-01), Ashley et al.
patent: 6583290 (2003-06-01), Ziermann et al.
patent: 6589968 (2003-07-01), Arslanian et al.
patent: 20030045711 (2003-03-01), Ashley et al.
patent: 20030073205 (2003-04-01), Arslanian et al.
patent: 20030096381 (2003-05-01), Julien et al.
patent: 41 38 042 (1993-05-01), None
patent: 0 423 714 (1991-04-01), None
patent: 0 428 169 (1991-05-01), None
patent: WO 9310121 (1993-05-01), None
patent: WO 9702358 (1997-01-01), None
patent: WO 9713845 (1997-04-01), None
patent: WO 9719086 (1997-05-01), None
patent: WO 9808849 (1998-03-01), None
patent: WO 9822461 (1998-05-01), None
patent: WO 9825929 (1998-06-01), None
patent: WO 9827203 (1998-06-01), None
patent: WO 9849315 (1998-11-01), None
patent: WO 9901124 (1999-01-01), None
patent: WO 9902514 (1999-01-01), None
patent: WO 9902669 (1999-01-01), None
patent: WO 9903986 (1999-01-01), None
patent: Wo 9907692 (1999-02-01), None
patent: WO 9943320 (1999-02-01), None
patent: WO 9927890 (1999-06-01), None
patent: WO 9939694 (1999-08-01), None
patent: WO 9940047 (1999-08-01), None
patent: WO 9942602 (1999-08-01), None
patent: WO 9943653 (1999-09-01), None
patent: WO 9954318 (1999-10-01), None
patent: WO 9954319 (1999-10-01), None
patent: WO 9954330 (1999-10-01), None
patent: WO 9965913 (1999-12-01), None
patent: WO 9966028 (1999-12-01), None
patent: WO 9967252 (1999-12-01), None
patent: WO 9967253 (1999-12-01), None
patent: WO 0000485 (2000-01-01), None
patent: WO 0001838 (2000-01-01), None
patent: WO 00 22139 (2000-04-01), None
patent: WO 0031247 (2000-06-01), None
patent: WO 01083800 (2001-11-01), None
patent: WO 02080846 (2002-10-01), None
An, J. and Kim, Y. (1998). “A Gene Cluster Encoding Malonyl-CoA Decarboxylase (MatA), Malonyl-CoA Synthetase (MatB) and a Putative Dicarboxylate Carrier Protein (MatC) inRhizobium trifolii,” Eur J Biochem274(52):395-402.
Arslanian, R.L. et al. (2002). “Large-Scale Isolation and Crystallization of Epothilone D fromMyxococcus xanthusCultures,”J. Natural Products65(4):570-572.
Beyer et al. (1999). “Metabolic Diversity in Myxobacteria: Identificaion of the Myxalamid and the Stigmatellin Biosynthetic Gene Cluster ofStigmatella auranticaSga15 and a Combined Polyketide-(poly)peptide Gene Cluster from the Epothilone Producing StrainSorangium cellulosumSo ce90,”Biochimica et Biophysica Acta1445(2):185-195.
Bretscher, A.P. et al. (1978). “Nutrition ofMyxococcus xanthus, a Fruiting Myxobacterium,”J. Bacteriology133(2):763-768.
Frykman, S. et al. (2002). “Modulation of Epothilone Analog Production Through Media Design,”J. Industrial Microbiology&Biotechnology28(1):17-20.
Hamilton, C. et al. (1989). “New Method for Generating Deletions and Gene Replacements inEscherichia coli,” J Bact171(9):4617-4622.
Honbo, T. et al. (1987). “The Oral Dosage Form of FK-506,”Transplantation ProceedingsXIX, 5(Suppl 6):17-22.
Jacobsen, J.R. et al. (1998). “Spontaneous Priming of a Downstream Module in 6-Deoxyerythronolide B,”Biochemistry37:4928-4934.
Jaoua, S. et al. (1992). “Transfer of Mobilizable Plasmids toSorangium cellosumand Evidence for Their Integration into the Chromosome,”0Plasmid28:157-165.
Lau, J. et al. (2002). “Optimizing the Heterologous Production of Epothilone D inMyxococcus xanthus,” Biotechnology and Bioengineering78(3):280-288.
Link, A. et al. (1997). “Methods for Generating Precise Deletions and Insertions in the Genome of Wild-TypeEscherichia coli: Application to Open Reading Frame Characterization,”J Bact179(20):6228-6237.
Nicolaou, K.C. et al. (1997). “Designed Epothilones: Combinatorial Synthesis, Tubulin Assembly Properties, and Cytotoxic Action Against Taxol-Resistant Tumor Cells,”Angew Chem Int Ed Engl36(19):2097-2103.
Nicolaou, K.C. et al. (1998). “Chemical biology of epothilones,”Angew Chem Int Ed37(15):2014-2045.
Oliynyk et al. (1996). “A Hybrid Modular Polyketide Synthase Obtained by Domain Swapping,”Chemistry&Biology3:833-839.
Paitan et al. (1999). “The First Gene in the Biosynthesis of the Polyketide Antibiotic TA ofMyxococcus xanthusCodes for a Unique PKS Module Coupled to a Peptide Synthetase,”J. Molecular Biology286:465-474.
Pfeifer, B.A. et al. (2001). “Biosynthesis of polyketides in heterologous hosts,”Microbiology and Molecular Biology Reviews65(1):106-118.
Regentin, R. et al. (2001). “Development of a cost effective epothilone D process inMyxococcus xanthus,” Abstracts of Papers American Chemical Society221(1-2): BIOT 61.
Shimkets, L.J. (1993). “Chapter 12: Industrial relevance and genetic analysis of myxobacteria,”Industrial Microorganisms: Basic and Applied Molecular Genetics5thASM pp. 85-96.
Silakowski, B. et al. (1999). “New Lessons for Combinatorial Biosynthesis From Myxobacteria: The Myxothiazol Biosynthetic Gene Cluster ofStigmatella AurantiacaDW4/3-1.”J Biol. Chem274(52):37391-37399.
Strong, S. et al. (1997). “Marked Improvement of PAC and BAC Cloning is achieved Using Electroelution of Pulsed-Field Gel-Separated Partial Digests of Genomic DNA,”Nucleic Acids Res19:3959-3961.
Ueki, T. et al. (1996). “Positive-Negative KG Cassettes for Construction of Multi-Gene Deletions Using a Single Drug Marker,” Gene 183:153-157.
Varon et al. (1992). “Mutation and Mapping of Genes Involved in Production of the Antibiotic TA inMyxococcus xanthus,” Antimicrobial Agents and Chemotherapy36(10):2316-2321.
Balog D., et al. (1996).Angew Chem Int Ed Engl35 (23/24):2801-2803.
Balog, D. et al., (1998).Angew Chem Int Ed Engl37(19):2675-2678.
Betlach, et al. (1998).Biochem37:14937.
Bierman, et al. (1992).Gene116:43-49.
Bollag, D. et al. (1995).Cancer Res55:2325-2333.
Campos ans Zusman, (1975).Proc Natl Acad Sci USA72:518-522.
Campos, et al. (1978).J Mol Biol119:167-168.
Caspers, et al.(1994).Cellular and Molecular Biology40(5):635-644.
Chou, T.C. et al. (1998).Natl Acad Sci USA95 (16):9642-9647.
Gerth, K. et al. (1996).J. Antibiotics49:560-563.
Hahn D., et al.. (1991).J. Bact173:5573-5577.
Hodgkin and Kaiser. (1979).Mol Gen Genet171:177-191.
Hofle, et al. (1996).Angew Chem Int Ed Engl35(13/14):1567-1569.
Jacobsen, et al. (1998).Biochemistry37:4928-4934.
Jaoua, et al. (1992).Plasmid28:157-165.
Kafeshi, et al. (1995).Mol Microbiol15:483-494.
Kaiser, (1979). Proc.Natl Acad Sci USA76:5952-5956.
Katz, et al. (1983).J Gen Micr

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