Cleaning compositions for solid surfaces – auxiliary compositions – Cleaning compositions or processes of preparing – Enzyme component of specific activity or source
Patent
1995-06-15
1998-03-24
Lieberman, Paul
Cleaning compositions for solid surfaces, auxiliary compositions
Cleaning compositions or processes of preparing
Enzyme component of specific activity or source
510530, 435198, 435200, 435201, 435202, 435203, 435204, C11D 3386, C12N 932
Patent
active
057312800
DESCRIPTION:
BRIEF SUMMARY
CROSS REFERENCE TO RELATED APPLICATIONS
This application is a continuation of PCT/DK93/00441 filed Dec. 22, 1993, which is incorporated herein by reference.
TECHNICAL FIELD
The present invention relates to lipase and .alpha.-amylase variants, stabilized towards the inactivation caused by peroxidase systems, in which lipase and .alpha.-amylase variants a naturally occurring tyrosine residue has been deleted or substituted with a different amino acid residue at one or more positions.
The invention also relates to a method of stabilizing a lipase or an .alpha.-amylase towards the inactivation caused by peroxidase systems, and detergent compositions comprising a lipase and/or an .alpha.-amylase variant of the invention.
BACKGROUND ART
Peroxidases (E.C. 1.11.1.7) are enzymes that catalyse the oxidation of a substrate (an electron or hydrogen donor) with hydrogen peroxide. Such enzymes are known from microbial, plant and animal origins, e.g. peroxidase from Coprinus cinereus (cf. e.g. EP Patent Application 179,486). They are typically hemoproteins, i.e. they contain a heme as a prosthetic group.
Use of peroxidase together with hydrogen peroxide or a hydrogen peroxide precursor has been suggested e.g. in bleaching of pulp for paper production, in treatment of waste water from pulp production, for improved bleaching in laundry detergents, for dye transfer inhibition during laundering, and for lignin modification, e.g. in particle board production.
Peroxidase systems (also designated POD systems) comprising a peroxidase or a compound exhibiting peroxidase activity, a source of hydrogen peroxide, and a peroxidase enhancing agent, are used for preventing coloured substances, which leach from dyed fabrics, to deposit on other fabrics present in the same wash (this phenomenon is commonly known as dye transfer). Detergent compositions or wash liquors comprising such peroxidase systems have been described in e.g. International Patent Applications WO 92/18687 and WO 92/18683.
A major drawback in applying such peroxidase systems to detergent compositions is that the enzymes present in such compositions may be strongly affected by the peroxidase system, thereby hampering the washing performance of the detergent composition.
SUMMARY OF THE INVENTION
It has now surprisingly been found that lipases and .alpha.-amylases may be stabilized towards inactivation caused by peroxidase systems, by deletion or substitution of one or more naturally occurring tyrosine residues with a different amino acid residue.
Accordingly, the invention provides a lipase and/or an .alpha.-amylase variant, in which one or more naturally occurring tyrosine residues have been deleted or substituted with a different amino acid residue.
In another aspect, the invention provides a method of stabilization of a lipase and/or an .alpha.-amylase variant towards inactivation caused by a peroxidase system, in which method one or more naturally occurring tyrosine residues are deleted or substituted with a different amino acid residue.
In a further aspect, the invention provides detergent compositions comprising a lipase and/or an .alpha.-amylase variant of the invention.
In a yet further aspect, the invention provides detergent additives comprising a lipase and/or an .alpha.-amylase variant of the invention.
DETAILED DISCLOSURE OF THE INVENTION
The present invention provides novel lipase and .alpha.-amylase variants, stabilized towards inactivation caused by peroxidase systems.
In the context of this invention, a stabilized lipase or .alpha.-amylase variant is a lipase or an .alpha.-amylase having improved stability towards inactivation caused by peroxidase systems, when compared to the parent lipase or .alpha.-amylase.
As abbreviations for amino acids the following symbols are used:
______________________________________ A = Ala = Alanine
C = Cys = Cysteine
D = Asp = Aspartic acid
E = Glu = Glutamic acid
F = Phe = Phenylalanine
G = Gly = Glycine
H = His = Histidine
I = Ile = Isoleucine
K = Lys = Lysine
L = Leu = Leucine
M = Met
REFERENCES:
patent: 4810414 (1989-03-01), Huge-Jensen
patent: 5030240 (1991-07-01), Wiersema et al.
patent: 5108457 (1992-04-01), Poulose et al.
Halkier Torben
Nielsen Egon
Rasmussen Grethe
Agris, Esq. Cheryl H.
Fries Kery A.
Lieberman Paul
Novo Nordisk A S
Zelson Esq. Steve T.
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