Recombinant L-N-carbamoylase from Arthrobacter aurescens and...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing alpha or beta amino acid or substituted amino acid...

Reexamination Certificate

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C435S004000, C435S006120, C435S069100, C435S183000, C435S200000, C435S228000, C435S107000, C435S108000, C435S109000, C435S115000, C435S116000, C536S023200, C536S023500, C536S023700

Reexamination Certificate

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06953677

ABSTRACT:
A novel rec-L-N-carbamoylase fromArthrobacter aurescensand its method of use for producing L-amino acids. The recombinantly produced L-carbamoylase is unexpectedly stable, so that an industrial method of producing L-amino acids can be established with it, in contrast to previously known L-carbamoylases.

REFERENCES:
patent: 5516660 (1996-05-01), Wagner et al.
patent: 0 625 571 (1994-11-01), None
patent: 2 728 905 (1996-07-01), None
Gross et al. (J. Biotechnol., 1990, vol. 14(3-4):363-375).
Wilms et al., “Cloning, nucleotide sequence and expression of a new L-N-carbamoylase gene from Arthrobacter aurescens DSM 3747 in E. coli”, Journal of Biotechnology, vol. 68, 1999, p. 101-113.
Siemann et al., “Detection and Comparison of strains with selective I-hydantoin cleaving activity using polyclonal antibodies”, Biotechnology Tecniques, vol. 7, No. 5, 1993, p. 361-366.
Gross et al., “Screening method for microorganisms producing L-aminoacids from D, L-5-monosubstituted hydantoins”, Biotechnology Techniques, vol. 1, No. 2, 1987, p. 85-90.
English language abstract of JR above.
English language abstract of KR above.

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