Recombinant human serum albumin, process for producing the same

Details Recombinant human serum albumin, process for producing the same Recombinant human serum albumin, process for producing the same

1993-03-24

1995-08-08

Beisner, William H.

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Albumin

530364, 530412, 530414, 530415, 530416, C07K 14765, C07K 116, C07K 136, A61K 3838

Patent

active

054400180

ABSTRACT:
Human serum albumin obtained by gene manipulation techniques can be purified by a combination of specified steps in which a culture supernatant obtained from a human serum albumin-producing host is subjected to ultrafiltration, heat treatment, acid treatment and another ultrafiltration, followed by subsequent treatments with a cation exchanger, a hydrophobic chromatography carrier and an anion exchanger, and by salting-out to thereby obtain a pure form of human serum albumin which contains substantially no proteinous and polysaccharide contaminants, which is formulated into a pharmaceutical preparation. This process makes it possible to effeciently purify recombinant human serum albumin and to provide substantially pure human serum albumin which does not contain producer host-related substances and other contaminants and is sufficiently free from coloration.

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patent: 4677027 (1987-06-01), Porath et al.
patent: 4990447 (1991-02-01), Konig et al.
patent: 5250663 (1993-10-01), Tenold
A. S. Michaels "New Separation Technique for the CPI" Chemical Engineering Progress (vol. 64 #12) Dec. 1968 pp. 31-43.
E L V Harris "Protein Purification Methods" 1989. Pp. 151-157, 175-177, 232-235.
Kagaku Kogyo Nippo, Jul. 30, 1992.

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