Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1988-08-30
1992-01-21
Weimar, Elizabeth C.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
4351723, 4352402, 4353201, 935 11, 935 22, 935 70, C12N 2106, C12N 1500, C12N 500
Patent
active
050827740
ABSTRACT:
An expression vector replicable in a mammalian cell includes a prepro-NGF encoding DNA sequence operably linked with regulatory DNA capable of effecting the expression of the prepro-NGF encoding DNA sequence in the cell. Also, a mammalian cell is transfected with such an expression vector. Also, a method for making recombinant human nerve growth factor includes transfecting a mammalian cell with such an expression vector, maintaining the cell in a culture medium, and isolating recombinant human nerve growth factor from the medium. Also, a composition includes recombinant human nerve growth factor made according to such a method combined with a pharmaceutically acceptable carrier.
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Hallbook, et al., "Production and Characterization of Biologically Active Recombinant Beta Nerve Growth Factor," Molecular and Cellular Biology, Jan. 1988, pp. 452-456.
Ullrich, et al., "Human B-Nerve Growth Factor Gene Sequence Highly Homologous to that of Mouse," Nature, 303:821-825, Jun. 1983.
Kaufman, et al., "Amplification and Expression of Sequences Cotransfected with a Modular Dihydrofolate Reductase Complementary DNA Gene," J. Mol. Biol. (1982) 159:601-621.
Lusky, et al., "Characterization of the Bovine Papilloma Virus Plasmid Maintenance Sequences," Cell, Jan. 1984, 36:391-402.
Kaufman, "Identification of the Components Necessary for Adenovirus Translational Control and their Utilization in cDNA Expression Vectors," Proc. Natl. Acad. Sci. USA, 82:689-693, Feb. 1985.
Chambers Jasemine C.
The General Hospital Corporation
Weimar Elizabeth C.
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