Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1995-06-05
1997-08-26
Jacobson, Dian C.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
4351723, 435325, 4353201, 435358, 530383, 536 235, C07K 14755, C12N 510, C12N 1512, C12N 1563
Patent
active
056610084
ABSTRACT:
A DNA sequence coding for a biologically active recombinant human factor VIII derivative, comprising a first DNA segment coding for the amino acids 1 through 740 of human factor VIII and a second DNA segment coding for the amino acids 1649 through 2332 of human factor VIII, said segments being interconnected by a linker DNA segment coding for a linker peptide of at least 3 amino acid residues and up to about 10 amino acid residues which are selected from lysine and arginine; recombinant expression vector comprising such DNA sequence; host cells of animal origin transformed with such recombinant expression vector; a process for the manufacture of recombinant human factor VIII derivative; and human factor VIII derivative containing the heavy chain and the light chain linked by metal ion bond.
REFERENCES:
patent: 4769326 (1988-09-01), Rutter
patent: 4868112 (1989-09-01), Toole
Proc. Natl. Acad. Sci. U.S.A., "Isolation and characterization of human factor VIII: Molecular forms in commercial factor VIII concentrate, cryoprecipitate, and plasma", vol. 83, pp. 2979 to 2983 (May 1986), L.O. Andersson et al.
Journal of Biological Chemistry, "The Functional Domains of Coagulation Factor VIII:C", vol. 261, No. 27, pp. 12574 to 12578 (1986), Rae Lyn Burke et al.
Biochemistry, vol. 25, No. 26 (Dec. 30, 1986), "Construction and Characterization of an Active Factor VIII Variant Lacking the Central One-Third of the Molecule", Dan L. Eaton, et al.
"Expression of Active Human Factor VIII From Recombinant DNA Clones", William I. Wood et al, Nature vol. 312, 22 Nov. 1984, pp. 330-337.
"Structure of Human Factor VIII", Gordon A. Vehar et al, Nature vol. 312, pp. 337-342 (1984).
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"A Large Region ( 95 kDa) of Human Factor VIII is Dispensable for In Vitro Procoagulant Activity", John J. Toole et al, Proc. Natl. Acad. Sci. UA, vol. 83, pp. 5939-5942, Aug. 1986.
"Stable Expression of Recombinant Factor VIII Molecules Using a Bovine Papillomavirus Vector", Nava Sarver et al, DNA, vol. 6, No. 6, 1987, pp. 553-564.
"Synthesis of Biologically Active Deletion Mutants of Human Factor VIII:C", K.D. Langner et al, Behring Inst. Mitt., No. 82, 16-25 (1988).
"A New Recombinant Procoagulant Protein Derived from the cDNA Encoding Human Factor VIII", Pierre Meulien et al, Protein Engineering, vol. 2, No. 4, pp. 301-306, 1988.
"Two Independent Domains of Factor VIII Co-Expressed Using Recombinant Vaccinia Viruses Have Procoagulant Activity", Andrea Pavirani et al, Biochemical and Biophysical Research Communications, vol. 145, No. 1, 1987, May 29, 1987, pp. 234-240.
Almstedt Annelie B.
Gray (Hellstrom) Eva Maria
Lind Peter
Ljung Catherine
Sandberg Helena Inga
Jacobson Dian C.
Kabi Pharmacia AB
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