Recombinant glucose uptake system

Details Recombinant glucose uptake system Recombinant glucose uptake system

1994-03-28

1997-02-11

Jacobson, Dian C.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

435 691, 435194, 43525233, 4352541, 43525411, 4352551, 4352552, 4353201, C12N 912, C12N 1554, C12N 121, C12N 1563

Patent

active

056020309

ABSTRACT:
Recombinant organisms are disclosed that contain a pathway for glucose uptake other than the pathway normally utilized by the host cell. In particular, the host cell is one in which glucose transport into the cell normally is coupled to PEP production. This host cell is transformed so that it uses an alternative pathway for glucose transport that is not coupled to PEP production. In a preferred embodiment, the host cell is a bacterium other than Z. mobilis that has been transformed to contain the glf and glk genes of Z. mobilis. By uncoupling glucose transport into the cell from PEP utilization, more PEP is produced for synthesis of products of commercial importance from a given quantity of biomass supplied to the host cells.

REFERENCES:
Ko et al., "Roles of Multiple Glucose Transporters in Saccharomyces cerevisiae," Molecular and Cellular Biology, Jan. 1993 vol. 13, No. 1, pp. 638-648.
Fukuda et al., "Cloning of Glucose Phosphorylating Genes in S. cerevisiae by the KU-Method and Application to ATP Production," Agric. Biol. Chem. vol. 48, No. 11, pp. 2877-2881, 1984.
Bisson et al., "Involvement of kinases in glucose and fructose uptake by Saccharomyces cerevisiae," Proc. Natl. Acad. Sci. USA, vol. 80, pp. 1730-1734, Mar. 1983.
Barnell, et al. Jrnl. of Bacteriology, "Sequence and Genetic Organization of a Zymomonas mobilis. . . ", 172:7227-7240 (Dec. 1990).
Fukuda et al. Jrnl. of Bacteriology, "Cloning of the Glucokinase Gene in Escherichia coli B", 156:922-925 (Nov. 1983).
Curtis et al. Jrnl. of Bacteriology, "Phosphorylation of D-Glucose in Escherichia coli Mutants Defective in . . . ", 122:1189-1199 (Jun. 1975).
Erni FEMS Microbiology Reviews, "Glucose transport in Escherichia coli", 63:13-24 (1989).
Postma "Phosphotransferase System for Glucose and . . . " in Escherichia Coli and Salmonella Typhimurium: Cellular and Molecular Biology, Neidhardt et al. (eds.) pp. 127-141 (1987).
Meadow et al. Annu. Rev. Biochem., "The Bacterial Phosphoenolpyruvate: Glucose Phosphotransferase System", 59:497-542 (1990).

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