Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Lymphokines – e.g. – interferons – interlukins – etc.
Patent
1994-09-23
1996-11-12
Sayala, Chhaya D.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Lymphokines, e.g., interferons, interlukins, etc.
424 855, 435 6951, 435811, C07K 1457
Patent
active
055741371
ABSTRACT:
The present invention relates to novel, stable recombinant gamma interferons exhibiting in greater or less degree the antiviral and antiproliferative activity in humans and pH 2 labile properties characteristic of native human gamma interferon. The amino acid sequence of such an interferon comprises, from the N terminus: ##STR1## wherein X is a methionine residue or hydrogen and Y is a glutamine residue or, where X is hydrogen, Y is either a glutamine or a pyroglutamate residue.
REFERENCES:
patent: 3981991 (1976-09-01), Stewart et al.
patent: 4017600 (1977-04-01), Stewart et al.
patent: 4311639 (1982-01-01), Ganfield et al.
patent: 4341761 (1982-07-01), Ganfield et al.
patent: 4361509 (1982-11-01), Zimmerman et al.
patent: 4388234 (1983-06-01), Horecker
patent: 4404188 (1983-09-01), Donahoe et al.
patent: 4432895 (1984-02-01), Tarnowski
patent: 4476049 (1984-10-01), Kung
patent: 4485017 (1984-11-01), Tan et al.
patent: 4681930 (1987-07-01), Kung et al.
patent: 5278286 (1994-01-01), Kung et al.
Hochkeppel, "Monoclonal antibody against human IFN-.gamma.", Nature 296:258-259 (1982).
Secher, et al., "A monoclonal antibody for large-scale purification of human leukocyte interferon", Nature 285:446-450 (1980).
Kung, et al., "Purification of Recombinant Human Immune Interferon" in Pestka, Interferons, Methods in Enzymology 119:204-210 (1986).
Gray, et al., "Expression of human immune interferon cDNA in E. coli and monkey cells", Nature 295:503-508 (1982).
Wetzel, et al., "Expression in Escherichia coli of a chemically synthesized gene for a `mini-C` analog of human proinsulin", Gene 16:63-71 (1981).
Wetzel, et al., "Production of Biologically Active N.sup..alpha. -Desacetylthymosin .alpha..sub.1 in Escherichia coli through Expression of a Chemically Synthesized Gene", Biochemistry 19:6096-6104 (1980).
Ullmann, et al., "On the Effect of Divalent Cations and Protein Concentration Upon Renaturation of .beta.-Galactosidase from E. coli", Biochemical and Biophysical Reserch Communications 35(1):35-42 (1969).
Staehelin, et al., "Production of hybridomas secreting monoclonal antibodies to the human leukocyte interferons", Proc. Natl. Acad. Sci. USA 78(3):1848-1852 (1981).
Staehelin, et al., "Purification and Characterization of Recombinant Human Leukocyte Interferon (IFLrA) with Monoclonal Antibodies", The Journal of Biological Chemistry 256(18):9750-9754 (1981).
Rehberg, et al., "Specific Molecular Activities of Recombinant and Hybrid Leukocyte Interferons", The Journal of Biological Chemistry 257(19):11497-11502 (1982).
Prouty, et al., "Degradation of Abnormal Proteins in Escherichia coli", The Journal of Biological Chemistry 250(3):1112-1122 (1975).
Livingston, "Immunoaffinity Chromatography of Proteins" in Affinity Techniques, Methods in Enzymology XXXIV:722-730 (1974).
Hatefi, et al., "Destabilization of Membranes with Chaotropic Ions" in Biomembranes, Methods in Enzymology XXXI:770-790 (1974).
Hager, et al., "Elution of Proteins from Sodium Dodecyl Sulfate-Polyacrylamide Gels, Removal of Sodium Dodecyl Sulfate, and Renaturation of Enzymatic Activity: Results with Sigma Subunit of Escherichia coli RNA Polymerase, Wheat Germ DNA Topoisomerase, and Other Enzymes", Analytical Biochemistry 109:76-86 (1980).
Altrock, et al., "Use of a Non-Neutralizing Synthetic Peptide Monoclonal Antibody for Quantitation and Characterization of Human IFN-.gamma. Analogs", Elsevier Science Publishers B. V., The Biology of the Interferon System, E. DeMaeyer and H. Schellekens, editors, pp. 135-138 (1983).
Sofer, et al., "Designing and Optimal Chromatographic Purification Scheme for Proteins", BioTechniques 1(4):198-203 (1983).
Bonnerjea, et al., "Protein Purification: The Right Step at the Right Time", Bio/Technology 4:954-958 (1986).
Marglin, et al., "Chemical Synthesis of Peptides and Proteins", Annual Review of Biochemistry 39:841-866 (1970).
Tarnowski, "Practical aspects in the recovery of proteins produced by recombinant DNA technology," Pharmaceutical Technology, Feb. 1983, pp. 70-79.
Blackburn in "Enzyme Structure and Function" Marcel Dekker, Inc., New York (1976) pp. 167-223.
Gray Patrick W.
Rinderknecht Ernst H.
Dreger Walter H.
Genentech Inc.
Sayala Chhaya D.
LandOfFree
Recombinant gamma interferons having enhanced stability and meth does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Recombinant gamma interferons having enhanced stability and meth, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Recombinant gamma interferons having enhanced stability and meth will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-563657