Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Reexamination Certificate
2003-08-22
2008-11-11
Mondesi, Robert B (Department: 1645)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
C435S320100, C435S252300, C435S071100, C435S069300, C435S069100, C530S350000, C536S023700, C536S023100, C424S236100, C424S234100, C424S184100
Reexamination Certificate
active
07449310
ABSTRACT:
TheF. necrophorum geneexpressing leukotoxin was sequenced and cloned. The leukotoxin open reading frame (lktA) is part of a multi-gene operon containing 9,726 bp, and encoding a protein containing 3,241 amino acids with an overall molecular weight of 335,956 daltons. The protein encoded by the gene was truncated into five polypeptides having overlapping regions by truncating the full length gene into five different sections and amplifying, expressing, and recovering the protein encoded by each of these sections. Additionally, a region upstream of the gene was sequenced and the polypeptide encoded by that nucleotide sequence was purified and isolated. These polypeptides along with the full length protein are then tested to determine their immunogenicity and protective immunity in comparison to the efficacy of immunization conferred by inactivated native leukotoxin inF. necrophorumculture supernatant.
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Testing of Combined Fusobacterium Necrophorum Leukotoxoid and Ac
Chengappa Muckatira M.
Nagaraja Tiruvoor G.
Narayanan Sanjeev K.
Stewart George C.
Baskar Padma V
Kansas State University Research Foundation
Mondesi Robert B
Truitt Tracey S.
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