Recombinant fusion proteins

Details Recombinant fusion proteins Recombinant fusion proteins

1992-03-19

2000-01-04

Fox, David T.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 691, 435 694, 435 696, 435 711, 4352523, 4353201, 435471, 536 234, 536 235, 536 2351, 536 2353, C12N 1562, C12N 1574, C12N 1512, C12N 1513, C12N 1514

Patent

active

060108835

ABSTRACT:
This invention provides a DNA sequence coding for a cleavage site which is specifically cleaved by blood coagulation Factor Xa, a vector containing such a sequence, and a host organism transformed with such a vector. Preferably, in the vector, the Factor Xa cleavage site coding sequence is fused at one end to a product and at its other end to an ATG codon or a sequence coding for at least part of a host protein.
This invention also provides a process, for the production of a desired protein or peptide product in native form, comprising:

REFERENCES:
patent: 4366246 (1982-12-01), Riggs
patent: 4532207 (1985-07-01), Brewer et al.
patent: 4543329 (1985-09-01), Daum et al.
Biological Abstracts, 63, 3, 1270, No. 12884 (1977).
Biological Abstracts, 74, 11, 7609, No. 73568 (1982).
Nagai, Nature, 309, 5971, pp. 810-812 (1984).
Sassenfeld et al., Bio/Technology, 2, 76-81 (1984).
"Protein Purification from Molecular Mechanisms to Large-Scale Processes," American Chemical Society, Washington, D.C. (1990), Chapter 13, pp. 181-193.
"Factor Xa for Recombinant Peptide Production --Specific Cleavage Releases Desired Product from Fusion Proteins" InFerGene COmpany, Benicia, California.
Aurell et al. 1977. Thrombosis Res. 11: 595-609.
Fujikawa et al. 1972. Biochem. 11(26): 4882-4891.
Degen et al. 1983. Biochem. 22: 2087-2097.
Herrera-Estrella et al. 1983. Nature 303: 209-213.

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