Recombinant expression of heterologous nucleic acids in...

Details Recombinant expression of heterologous nucleic acids in... Recombinant expression of heterologous nucleic acids in...

2005-01-25

2005-01-25

Navarro, Mark (Department: 1645)

Drug, bio-affecting and body treating compositions

Whole live micro-organism, cell, or virus containing

C424S093200, C424S093210, C435S069100, C435S252300, C435S320100, C435S325000

Reexamination Certificate

active

06846481

ABSTRACT:
The ciliated protozoan Tetrahymena exemplifies a recombinant system for the expression of heterologous nucleic acids, preferably on the plasma membrane surface. Integration of a heterlogous nucleic acid into the β-tubulin gene, BTU1, of a paclitaxel-sensitiveT. thermophilamutant that possesses btu1-1K350M β-tubulin allele allows screening for transformants using negative selection, as transformants have restored paclitaxel resistance. Transgenic ciliated protozoa of the invention can serve as live vaccines. For example, transgenic Tetrahymena expressingIchthyophthirius multifiliisi-antigen protein on their surface are effective vehicles for vaccination of freshwater fish against infection byI. multifiliis.

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