Recombinant expression cassettes with a fungal 3′...

Details Recombinant expression cassettes with a fungal 3′... Recombinant expression cassettes with a fungal 3′...

2007-12-19

2010-12-14

Collins, Cynthia (Department: 1638)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C435S320100, C435S419000, C800S298000, C800S312000, C800S314000, C800S320100

Reexamination Certificate

active

07851611

ABSTRACT:
The present invention provides recombinant expression cassettes comprising a fungal 3′ termination sequence which is functional in a plant. The recombinant expression cassettes comprise a plant promoter operably linked to a coding sequence having a stop codon, and the fungal termination sequence. The fungal 3′ termination sequence is heterologous to the coding sequence. The fungal 3′ termination sequence comprises structural features including a cleavage site, a positioning element and an upstream element. The present invention also comprises methods for construction of the plant expression cassettes and introducing the cassettes into plant cells.

REFERENCES:
patent: 7344885 (2008-03-01), Wilkinson et al.
Gillespie D. The magic and challenge of DNA probes as diagnostic reagents. Vet Microbiol. Sep. 1990;24(3-4):217-33. Review, p. 229.
Rothnie H.M et al. The contribution of AAUAAA and the upstream element UUUGUA to the efficiency of mRNA 3′-end formation in plants. EMBO J. May 1, 1994;13(9):2200-10.
Rothnie, H.M., et al.; “The contribution of AAUAAA and the upstream element UUUGUA to the efficiency of mRNA 3′-end formation in plants.”; EMBO J. (May 1, 1994) 13(9):2200-10; IRL Press Limited, Oxford, UK.

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