Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Reexamination Certificate
2011-03-01
2011-03-01
Collins, Cynthia (Department: 1638)
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
C435S320100, C435S419000, C800S298000, C800S312000, C800S314000, C800S320100
Reexamination Certificate
active
07897745
ABSTRACT:
The present invention provides recombinant expression cassettes comprising a fungal 3′ termination sequence which is functional in a plant. The recombinant expression cassettes comprise a plant promoter operably linked to a coding sequence having a stop codon, and the fungal termination sequence. The fungal 3′ termination sequence is heterologous to the coding sequence. The fungal 3′ termination sequence comprises structural features including a cleavage site, a positioning element and an upstream element. The present invention also comprises methods for construction of the plant expression cassettes and introducing the cassettes into plant cells.
REFERENCES:
patent: 7344885 (2008-03-01), Wilkinson et al.
Gillespie D. The magic and challenge of DNA probes as diagnostic reagents. Vet Microbiol. Sep. 1990;24(3-4):217-33. Review, p. 229.
Rothnie H.M et al. The contribution of AAUAAA and the upstream element UUUGUA to the efficiency of mRNA 3′-end formation in plants. EMBO J. May 1, 1994;13(9):2200-10.
Rothnie, H.M., et al.; “The contribution of AAUAAA and the upstream element UUUGUA to the efficiency of mRNA 3′-end formation in plants.”; EMBO J. (May 1, 1994) 13(9):2200-10; IRL Press Limited, Oxford, UK.
Bertain Sean
McBride Kevin
Wilkinson Jack Q.
Collins Cynthia
Pioneer Hi Bred International Inc.
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