Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound having a 1-thia-5-aza-bicyclo
Patent
1992-08-28
1994-06-07
Baker, Keith
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound having a 1-thia-5-aza-bicyclo
435 51, 435183, 435230, 435935, C12P 3502, C12P 3506, C12N 984
Patent
active
053188969
ABSTRACT:
An important intermediate for preparing cephalosporin antibiotics, 7-aminodesacetoxy cephalosporanic acid (7-ADCA), is prepared by a novel bioprocess in which a transformed Penicillium chrysogenum strain is cultured in the presence of an adipate feedstock to produce adipoyl-6-APA (6-amino penicillanic acid); and the in situ expression of an expandase gene, e.g., from Streptomyces clavuligerus, with which the P. chrysogenum has been transformed, converts the adipoly-6-APA by ring expansion to adipoyl-7-ADCA. The final product, 7-ADCA, is then prepared by cleavage of the adipoyl side chain using an adipoyl acylase. The entire synthesis, accordingly, is carried out using bioprocesses, and is efficient and economical.
REFERENCES:
patent: 5070020 (1991-12-01), Ingolia et al.
patent: 5108918 (1992-04-01), Groenen et al.
Baldwin et al., "The Enzymatic Ring Expansion of Penicillins to Cephalosporins: Side Chain Specificity", Tetrahedron, 43:3009-3014, (1987).
Ballio et al., "Incorporation of .alpha.,.omega.-Dicarboxylic Acids as Side-chains into the Penicillin Molecule", Nature, 185:97-99 (1960).
Cantwell et al., "Cloning and Expression of a hybrid S. clavuligerus cefE gene . . . ", Curr. Genet., 17:213-221 (1990).
Matsuda et al., "Cloning and Characterization of the Genes . . . ", J. Bacteriol., 169:5815-5820 (1987).
Conder Michael J.
McAda Phyllis C.
Rambosek John A.
Baker Keith
Matukaitis Paul D.
Merck & Co. , Inc.
Tribble Jack L.
Wallen III John W.
LandOfFree
Recombinant expandase bioprocess for preparing 7-aminodesacetoxy does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Recombinant expandase bioprocess for preparing 7-aminodesacetoxy, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Recombinant expandase bioprocess for preparing 7-aminodesacetoxy will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-792265