Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1995-09-14
1998-06-09
Hendricks, Keith D.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
435200, 4353201, 43525233, 43525231, 536 232, C12P 1912, C12N 924, C12N 1556
Patent
active
057632286
ABSTRACT:
A recombinant enzyme, having a molecular weight of about 57,000-67,000 daltons on SDS-PAGE and a pI of about 4.1-5.1 on isoelectrophoresis, which converts maltose into trehalose and vice versa. Depending on the enzymatic conditions, the enzyme forms about 70 w/w % of trehalose when acts on maltose, while about 20 w/w % of maltose when acts on trehalose. The culture of a transformant, prepared by introducing into a host a recombinant DNA containing a DNA coding for the enzyme and a self-replicable vector, facilitates the industrial-scale production of trehalose.
REFERENCES:
patent: 4521252 (1985-06-01), Miyake et al.
patent: 5538883 (1996-07-01), Nishimoto et al.
Nishimoto et al, "Existence of a novel enzyme converting Maltose into Trehalose", Bioscience Biotechnology and Biochemistry, vol. 59, No. 11, pp. 2189-2190, 1995.
Tsusaki et al, "Cloning and sequencing of Trehalose Synthase gene from primelobacter sp. R48", Biochimica et Biophysica Acta, vol. 1290, pp. 1-3, 1996.
Nishimoto et al, "Purification and characterization of a thermostable Trehalose Synthase from Thermus aquaticus", Bioscience Biotechnology and Biochemistry, vol. 60, No. 5, pp. 835-839, 1996.
Nishimoto et al, "Purification and properties of a novel enzyme, Trehalose Synthase, from Primelobacter sp. R48", Bioscience Biotechnology and Biochemistry, vol. 60, No. 4, pp. 640-644, 1996.
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J. Sambrook et al, Molecular Cloning, Cold Spring Harbor Laboratory Press, vols. 1, 2 and 3, 1989.
Kubota Michio
Sugimoto Toshiyuki
Tsusaki Keiji
Hendricks Keith D.
Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo
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