Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1995-12-19
1998-04-07
Wax, Robert A.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
435 691, 435 701, 435 703, 435 912, 435814, 536 232, C12N 948, C12P 2106, C12P 1934, C07H 2104
Patent
active
057363765
ABSTRACT:
Disclosed is a nucleic acid segment encoding endothelin converting enzyme-2 (ECE-2), that produces mature ET-1 from big ET-1 both in vitro and in transfected cells. Also disclosed is a polypeptide composition comprising partially purified ECE-2 and methods of using ECE-2 to screen candidate substances as inhibitors of ECE-2. Methods of producing recombinant ECE-2 are also disclosed.
REFERENCES:
patent: 5231166 (1993-07-01), Masaki et al.
patent: 5294569 (1994-03-01), Masaki et al.
patent: 5338726 (1994-08-01), Shiosaki et al.
patent: 5384243 (1995-01-01), Gutkind et al.
patent: 5427922 (1995-06-01), Fujisawa et al.
Ahn et al., "The Endothelin-Converting Enzyme from Human Umbilical Vein is a Membrane-Bound Metalloprotease Similar to that from Bovine Aortic Endothelial Cells", Proc. Natl. Acad. Sci. USA, 89:8606-8610, 1992.
Anderson and Orci, "A View of Acidic Intracellular Compartments", The Journal of Cell Biology, 106:539-543, 1988.
Arai et al., "Aspergillomarasmine A and B, Potent Microbial Inhibitors of Endothelin-Converting Enzyme", Biosci. Biotech. Biochem., 57(11):1944-1945, 1993.
Arai et al., "Cloning and Expression of a cDNA Encoding an Endothelin Receptor", Nature, 348:730-732, 1990.
Bax and Saxena, "The Current Endothelin Receptor Classification: Time for Reconsideration?", Elsevier Science LTD--TiPS, 15:379-386, 1994.
Baynash et al., "Interaction of Endothelin-3 with Endothelin-B Receptor Is Essential for Development of Epidermal Melanocytes and enteric Neurons", Cell, 79:1277-1285, 1994.
Clozel et al., "Pathophysiological Role of Endothelin Revealed by the First Orally Active Endothelin Receptor Antagonist", Nature, 365:759-761, 1993.
Douglas et al., "Novel Receptor Antagonists Welcome a New Era in Endothelin Biology", Elsevier Science Ltd--TiPS, 15:313-316, 1994.
Emoto and Yanagisawa, "Endothelin-Converting Enzyme-2 Is a Membrane-Bound, Phosphoramidon-Sensitive Metalloprotease with Acidic pH Optimum", The Journal of Biological Chemistry, 270(25):15262-15268, 1995.
Giaid et al., "Expression of Endothelin-1 in the Lungs of Patients with Pulmonary Hypertension", The New England Journal of Medicine, 328(24):1732-1739, 1993.
Hosada et al., "Targeted and Natural (Peibald-Lethal) Mutations of Endothelin B Receptor Gene Produce Megacolon Associated with Spotted Coat Color in Mice", Cell, 79:1267-1276, 1994.
Inoue et al., "The Human Endothelin Family: Three Structurally and Pharmacologically Distinct Isopeptides Predicted by Three Separate Genes", Proc. Natl. Acad. Sci. USA, 86:2863-2867, 1989.
Inoue et al., "The Human Preproendothelin-1 Gene", The Journal of Biological Chemistry, 264(25):14954-14959, 1989.
Kurihara et al., "Elevated Blood Pressure and Craniofacial Abnormalities in Mice Deficient in Endothelin-1", Nature, 368(21):703-710, 1994.
Lee et al., "Molecular Cloning and Primary Structure of Kell Blood Group Protein", Proc. Natl. Acad. Sci. USA, 88:6353-6357, 1991.
Matsumoto et al., "Abundance of Endothelin-3 in Rat Intestine, Pituitary Gland and Brain", Biochemical and Biophysical Research Communications, 164(1):74-80, 1989.
McMahon et al., "Phosphoramidon Blocks the Pressor Activity of Porcine Big Endothelin-1-(-139) in vivo and Conversion of Big Endothelin-1-(1-39) to Endothelin-1-(1-21) in vitro", Proc. Natl. Acad. Sci. USA, 88:703-707, 1991.
Nishikori et al., "Receptor Binding Affinity and Biological Activity of C-Terminal Elongated Forms of Endothelin-1", Neurochem.Int., 18(4):535-539, 1991.
Ohlstein et al., "SB 209670, A Rationally Designed Potent Nonpeptide Endothelin Receptor Antagonist", Proc. Natl. Acad. Sci. USA, 91:8052-8056, 1994.
Ohnaka et al., "Purification and Characterization of a Phosphoramidon-sensitive Endothelin-converting Enzyme inPorcine Aortic Endothelium," The Journal of Biological Chemistry, 268(35):26759-26766, 1993.
Okada et al., "Conversion of Big Endothelin-1 By Membrane-Bound Metalloendopeptidase in Cultured Bovine Endothelial Cells," Biochemical and Biophysical Research Communications, 171(3):1192-1198.
Okada, et al., "Big Endothelin-1 Structure Important for Specific Processing by Endothelin-Converting Enzyme of Bovine Endothelian Cells", Eur. J. Biochem., 218:493-498, 1993.
Opgenorth et al., "Endothelin-Converting Enzymes", The FASEB Journal, 6:2653-2659, 1992.
Rawlings and Barrett, "Evollutionary Families of Peptidases", Biochem J., 290:205-218, 1993.
Sakamoto et al., "Distinct Subdomains of Human Endothelin Receptors Determine Their Seklectivity to Endothelin.sub.A -Selective Antagonist and Endothelin.sub.b -Selective Agonists", The Journal of Biological Chemistry, 268(12);8547-8553, 1993.
Sakurai et al., "Cloning of cDNA Encoding a Non-Isopeptide-Selective Subtype of the Endothelin Receptor", Nature, 348:732-735, 1990.
Sawamura et al., "Phosphoramidon Inhibits the Intravellular Conversion of Big Endothelin-1 to Endothelin-1 in Cultured Endothelial Cells", Biochemical and Biophysical Research Communications, 174(2):779-784, 1991.
Seidah et al., "Mammalian Paired Basic Amino Acid Convertases of Prohormones and Proproteins.sup.a ", Annals of the New York Academy of Sciences, 680:135-146, 1993.
Shimada et al., "Cloning and Functional Expression of Endothelin-Converting Enzyme from Rat Endothelial Cells", The Journal of Biological Chemistry, 269(28):18275-18278, 1994.
Suzuki et al., "A Sensitive Sandwich-Enzyme Immunoassay for Human Endothelin", Journal of Immunological Methods, 118:245-250, 1989.
Takahashi et al., "Purification and Characterization of Endothelin-Converting Enzyme from Rat Lung", The Journal of Biological Chemistry, 268(28):21394-21398, 1993.
Turner, Anthony J., "Endothelin-converting Enzymes and Other Families of Metallo-endopeptidases", Biochemical Society Transactions, 21:697-701, 1993.
Vijayaraghavan et al., "The Hydrolysis of Endothelins by Neutral Endopeptidase 24.11 (Enkephalinase)," The Journal of Biological Chemistry, 265:(24)14150-14155.
Waxman et al., "Identification and Characterization of Endothelin Converting Activity from EAHY 926 Cells: Evidence for the Physiologically Relevant Human Enzyme," Archives of Biochemistry and Biophysics, 308(1):240-253, 1994.
Wilhelm et al., "Matrix Metalloproteinase-3 (Stromelysin-1)", The Journal of Biological Chemistry, 268(29):21906-21913, 1993.
Xu et al., "ECE: A Membrane-Bound Metalloprotease That Catalyzes the Proteolytic Activation of Big Endothelin-1", Cell, 78:473-485, 1994.
Yanagisawa et al., "A Novel Potent Vasoconstrictor Peptide Produced by Vascular Endothelial Cells", Nature, 332:411-415, 1988.
Yanagisawa, Masashi, "The Endothelin System: A New Target for Therapeutic Intervention", Editorial Circulation, 89(3):1320-1323, 1994.
Malfroy et al., "Molecular Cloning and Amino Acid Sequence of Human Enkephalinase (Neutral Endopeptidase", 229(1):206-210, 1988.
Board of Regents , The University of Texas System
Slobodyansky Elizabeth
Wax Robert A.
LandOfFree
Recombinant endothelin converting enzyme-2 and its use in ECE in does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Recombinant endothelin converting enzyme-2 and its use in ECE in, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Recombinant endothelin converting enzyme-2 and its use in ECE in will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-11935