Recombinant DNA, transformed host microorganism and method for p

Details Recombinant DNA, transformed host microorganism and method for p Recombinant DNA, transformed host microorganism and method for p

1988-09-12

1996-04-16

Naff, David M.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 712, 4351723, 435233, 4353201, 43525233, 435849, 536 231, 536 232, 930240, 935 14, 935 19, 935 23, 935 28, 935 41, 935 59, 935 61, 935 73, C12P 2106, C12N 1500, C12N 120, C07H 2104

Patent

active

055081762

ABSTRACT:
A polypeptide having mutarotase activity is obtained from a host microorganism that has been transformed with a molecule having a recombinant DNA sequence. The molecule having a recombinant DNA sequence is prepared by removing the first 60 nucleotides of a DNA sequence originating from the genome of Acinetobacter calcoaceticus that codes for the polypeptide, modifying the following 21 nucleotides and fusing of the resultant structural gene with the start of the tetracycline-repressor gene and with an effective promotor sequence. The tetracycline-regressor gene and the promotor sequence preferably originate from the same microorganism such as E. coli in which expression of the polypeptide is carried out, and result in increased yield of the expressed polypeptide having mutarotase activity.

REFERENCES:
patent: 4963488 (1990-10-01), Gatz et al.
"Principles of Gene Manipulation", Old & Primrose; Blackwell Scientific Publications; 1985.
Bachmair, A. et al., "In Vivo Half-life of a Protein Is a Function of Its Amino Terminal Residue", Science, vol. 234, pp. 179-186, 1986.
Gatz, C. et al. "Acinetobacter Calcoaceticus encoded Mutarotase: Nucleotide Sequence Analysis of a Gene and Characterization of its Secretion in E. coli," Nucleic Acids Research vol. 14, pp. 4309-4323 (1986).
Lambert, P. F. et al., "Use of Transcriptional Repressors to Stabilize Plasmid Copy Number of Transcriptional Fusion Vectors," J. Bacteriology vol. 162, 1, pp. 441-444 (1985).

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