Recombinant DNA, transformant containing said DNA, and process f

Details Recombinant DNA, transformant containing said DNA, and process f Recombinant DNA, transformant containing said DNA, and process f

1989-09-22

1992-05-19

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Oxidoreductase

536 27, 4353201, 43525233, 935 10, 935 73, C12N 904, C12N 1553, C12N 1570, C12N 121

Patent

active

051148536

ABSTRACT:
A recombinant DNA replicable and expressible in Escherichia coli is disclosed which encodes glucose dehydrogenase originating from Bacillus megaterium. A DNA sequence encoding the glucose dehydrogenase is incorporated into an E. coli vector and then used to transform an E. coli host. Also disclosed are transformants and recombinant DNA incorporating thermostable glucose dehydrogenase, wherein at least one amino acid in glucose dehydrogenase at positions 96, 252, and 253 is replaced by a thermostability-conferring amino acid.

REFERENCES:
patent: 3964974 (1976-06-01), Banauch et al.
patent: 4120755 (1978-10-01), Pierre et al.
patent: 4980288 (1990-12-01), Bryan et al.
Nagao, T. et al., 1989, FEBS Lett. vol. 253, pp. 113-116.
Heilmann, H. J. et al., 1988, Eur. J. Biochem., vol. 174, pp. 485-490.

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