Recombinant DNA-molecules and method for protein production

Details Recombinant DNA-molecules and method for protein production Recombinant DNA-molecules and method for protein production

1987-11-30

1991-04-23

Martinell, James

Chemistry: molecular biology and microbiology

Vector, per se

435 691, 435 6951, 435 697, 435 698, 435 712, 4351723, 435231, 43525231, 435320, 536 27, 935 48, 935 74, C12P 2100, C12N 1500, C12N 120, C12N 986

Patent

active

050100157

ABSTRACT:
The invention relates to recombinant DNA molecules and to methods for producing proteins by means of said molecules. Particularly, the present invention relates to recombinant DNA molecules which are synthetized in Bacillus strain bacteria and are characterized by DNA which codes for exoenzymes excreted by a bacterium of the Bacillus strain and which are present in tens of copies in Bacillus strain bacteria, as well as to recombinant DNA molecules which are modified from the above recombinant DNA molecules and are characterized by DNA containing the regulation and excretion signals of the .alpha.-amylase gene of B. amyloliquefaciens, to which signals a gene of any protein can be joined. These recombinant DNA molecules can be used, for example, to improve the production of .alpha.-amylase in Bacillus strain bacteria, and their modifications to produce any protein in Bacillus strain bacteria.

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