Recombinant DNA molecule

Chemistry: molecular biology and microbiology – Miscellaneous

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4351723, 435231, 935 48, 536 27, C12N 100, C12N 1500, C12N 986, C07H 1512

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045657858

ABSTRACT:
A plasmid or phage gene for a periplasmic or extracellular bacterial protein is cleaved, a double-stranded DNA sequence coding for a selected protein or portion thereof from a eukaryotic cell such as insulin is inserted in that cleaved gene by recombinant DNA techniques and used to transform a bacterium, and the excreted selected protein is collected.

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