Recombinant DNA methods vectors and host cells

Details Recombinant DNA methods vectors and host cells Recombinant DNA methods vectors and host cells

1995-01-23

1999-04-06

Ziska, Suzanne E.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

4353201, 435 691, 435696, 536 231, C12N 1500, C12P 2106, C12P 2104, C07H 2102

Patent

active

058916938

ABSTRACT:
The present invention relates to vectors useful for transforming a lymphoid cell line to glutamine independence. The vectors comprise an active glutamine synthetase (GS) gene as well as a heterologous gene of interest to be expressed. The preferred embodiments encompass vectors wherein the heterologous gene is expressed from a relatively strong promoter and the GS gene is expressed from a relatively weak promoter. In one example, the heterologous gene is operatively linked to the hCMV-MIE promoter and the GS gene is operatively linked to the SV40 early region promoter.

REFERENCES:
patent: 4399216 (1983-08-01), Axel et al.
patent: 4656134 (1987-04-01), Ringold
patent: 4797359 (1989-01-01), Finkelstein
patent: 5122464 (1992-06-01), Wilson
Foeching et al, Gene 45: 101, 1986.
Alberts et al., "Molecular Biology of the Cell," Garland Publishing, Inc., New York, pp. 184-193 (1983).
Watson et al., "Recombinant DNA, A Short Course," Freeman & Co., pp. 50-90 (1983).
Sanders, Peter G. et al., "Amplification and cloning of the Chinese hamster glutamine synthetase gene," The EMBO Journal, vol. 3, No. 1, pp. 65-71 (1984).
Pennica, Diane et al., "Cloning and expression of human tissue-type plasminogen activator cDNA in E. coli", Nature, vol. 301, pp. 214-221, Jan. 20, 1993.
Donn, Gunter et al., "Herbicide-Resistant Alfalfa Cells An Example of Gene Amplicicaton in Plants," Journal of Molecular and Applied Genetics, pp. 621-635 (1984).
Young, Anthony P. et al., "Mouse 3T6 Cells That Over-Produce Glutamine Synthetase," The Journal of Biological Chemistry, vol. 258, No. 18, pp. 11260-11266 (Sep. 25, 1983).
de Saint Vincent, Bruno Robert et al., "The Cloning and Reintroduction into Animal Cells of a Functional CAD Gene, a Dominant Amplifiable Genetic Marker," Cell, vol. 27, pp. 267-277 (Dec. 1981).
Murray, Mark J. et al., "Construction and Use of a Dominant, Selectable Marker: a Harvey Sarcoma Virus-Dihydrofolate Reductase Chimera," Molecular and Cellular Biology, vol. 3 , No. 1 pp. 32-43, Jan. 1983.
Kaufman, Randal J. et al., "Selection and amplification of heterologous genes encoding adenosine dreaminase in mammalian cells," Proc. Natl. Acad. Sci. USA, vol. 83, pp. 3136-3140 (May 1986).
Kaback, David B. et al., "Ribosomal DNA Magnification in Saccharomyces cerevisiae," Journal of Bacteriology, vol. 134, No. 1, pp. 237-245 (Apr. 1978).
Vel 'kov, V. V., "Amplification of Genes in Prokaryotic and Eukaryotic Systems," Soviet Genetics, vol. 18, pp. 384-396 (1982).
Sambrook, J. et al. Molecular Cloning A Laboratory Manual, Second Edition, Cold Spring Harbor Laboratory Press, pp. 16.45 to 16.46 (1989).
Foecking, Mary K. et al., "Powerful and versatile enhancer-promoter unit for mamalian expression vectors," Gene 45, pp. 101-105 (1986).
Review of Physiological Chemistry, 16th Edition, Chapter 23--p. 369, Lange Medical Publications, (1977).

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