Recombinant DNA methods, vectors and host cells

Details Recombinant DNA methods, vectors and host cells Recombinant DNA methods, vectors and host cells

1992-06-12

1999-03-09

Campell, Bruce R.

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

435375, C12N 500

Patent

active

058799362

ABSTRACT:
The present invention relates to vectors useful for transforming a lymphoid cell line to glutamine independence. The vectors comprise an active glutamine synthetase (GS) gene as well as a heterologous gene of interest to be expressed. The preferred embodiments encompass vectors wherein the heterologous gene is expressed from a relatively strong promoter and the GS gene is expressed from a relatively weak promoter. In one example, the heterologous gene is operatively linked to the hCMV-MIE promoter and the GS gene is operatively linked to the SV40 early region promoter.

REFERENCES:
patent: 4656134 (1987-04-01), Ringold
patent: 5122464 (1992-06-01), Wilson et al.
Rodrued, Chapter 23 p. 369, in Review of Physiological Chemistry, 1977, Lange Medical Publication.
Foecking et al Gene 45:101, 1986.
Molecular Cloning: A Laboratory Manual pp.1645-1646, 1989.

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