Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1991-01-17
1998-10-20
Wax, Robert A.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
435694, 4351723, 43525235, 4353201, 435 10, 435 29, 435 47, 435 60, 435 75, 514 29, 536 232, C12P 1962, A61N 31335, C12N 1552, C12N 1576
Patent
active
058245138
ABSTRACT:
A method to produce novel polyketide structures by designing and introducing specified changes in the DNA governing the synthesis of the polyketide is disclosed. The biosynthesis of specific polyketide analogs is accomplished by genetic manipulation of a polyketide-producing microorganism by isolating a polyketide biosynthetic gene-containing DNA sequence, identifying enzymatic activities associated within the DNA sequence, introducing one or more specified changes into the DNA sequence which codes for one of the enzymatic activities which results in an altered DNA sequence, introducing the altered DNA sequence into the polyketide-producing microorganism to replace the original sequence, growing a culture of the altered microorganism under conditions suitable for the formation of the specific polyketide analog, and isolating the specific polyketide analog from the culture. The method is most useful when the segment of the chromosome modified is involved in an enzymatic activity associated with polyketide biosynthesis, particularly for manipulating polyketide synthase genes from Saccarharopolyspora or Streptomyces.
REFERENCES:
patent: 4874748 (1989-10-01), Katz et al.
patent: 4935340 (1990-06-01), Baltz et al.
patent: 5081023 (1992-01-01), Yaginuma et al.
patent: 5087563 (1992-02-01), Beremand et al.
patent: 5110728 (1992-05-01), Kridl et al.
patent: 5141926 (1992-08-01), Weber et al.
patent: 5252474 (1993-10-01), Gewain et al.
Nature, vol. 348(8), pp. 176-178 (1990), Cortes, et al.
Gene, vol. 90, pp. 21-29 (1990), Tuan, et al.
Antimicrobial Agents and Chemotherapy vol. 34 (8), pp. 1535-1541 (1990), Huber, et al.
Molecular Genetics of Polyketides and its Comparison to Fatty Acid Biosynthesis, vol. 24, pp. 37-66 (1990), Hopwood, et al.
The EMBO Journal, vol. 8(9), pp. 2727-2736 (1989), Bibb, et al.
Journal of Bacteriology, vol. 171(11), pp. 5872-5881 (1989), Vara et al.
Molecular Microbiology, vol. 3(10), pp. 1405-1414 (1989), Dhillon, et al.
The EMBO Journal, vol. 8(9), pp. 2717-2725 (1989), Sherman, et al.
Antimicrobial Agents and Chemotherapy vol. 33(9), pp. 1413-1418 1989, Kirst, et al.
Genetics and Molecular Biology of Industrial Microorganism, pp. 53-59 (1989), Donadio, et al.
J. Chem. Soc. Chem. Commun. 943-945 (1988), Kinoshita, et al.
Antimicrobial Agents and Chemotherapy , vol. 32(10), pp. 1472-1476 (1988), Tomich, P.K.
Bio/technology, vol. 4, pp. 229-232 (1986), Stanzak, et al.
D.A. Hopwood, et al., "Production of `Hybrid` Antibiotics by Genetic Engineering", Nature, vol. 314, issued 18 Apr. 1985, pp. 642-644.
F. Malpartida, et al, "Homology Between Streptomyces Genes Coding for Synthesis of Different Polyketides Used to Clone Antibiotic Biosynthesis Genes", Nature, vol. 325, issued 26 Feb. 1987, pp. 818-821.
J.Mark Weber, et al, "Genetic Analysis of Erythromycin Production in Streptomyces Erythreus", Journal of Bacteriology, vol. 164, No. 1, issued Oct. 1985, pp. 425-433.
J.Mark Weber, et al, "Organization of a Cluster of Erythromycin Genes in Saccaropolyspora Erythraea", vol. 172, No. 5, issued May 1990, pp. 2372-2383.
Donadio Stefano
Katz Leonard
McAlpine James B.
Abbott Laboratories
Casuto Dianne
Danckers Andreas M.
Moore William W.
Wax Robert A.
LandOfFree
Recombinant DNA method for producing erythromycin analogs does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Recombinant DNA method for producing erythromycin analogs, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Recombinant DNA method for producing erythromycin analogs will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-243240