Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1995-02-07
1998-05-05
Vogel, Nancy T.
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
435326, 4353721, 435 691, 4353201, C12N 1500, C12N 506, C12N 1585, C12P 2106
Patent
active
057473080
ABSTRACT:
There is described a method for obtaining a eukaryotic cell containing in its DNA multiple copies of a GS gene, comprising: transforming a eukaryotic glutamine auxotroph with a GS gene; selecting transformant cells containing the GS gene; and culturing the selected transformant cells in a medium which lacks glutamine or in which the amount of glutamine is progressively depleted, the GS gene being of a character such that, or the conditions employed during the culturing step being such that, the GS gene is so weakly transcribed that the cells in which the GS gene has been amplified are selected.
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Roberts et al, "Gene Amplification and Gene Correction in Somatic Cells", Cell, 29:109-119, (1982).
Israel et al, "Highly Inducible Expression From Vectors Containing Multiple GRE's in CHO Cells Overexpressing The Glucocorticoid Receptor," Nucleic Acids Research, 17:4589-4603, (1989).
Bebbington et al, "The Use of Vectors Based on Gene Amplification For The Expression of Cloned Genes in Mammalian Cells," DNA Cloning, 3:163-188, (1987).
Hayward et al, "The Cloning And Nucleotide Sequence of CDNA For An Amplified Glutamine Synthetase Gene From The Chinese Hamster," Nucleic Acids Research, 14:999-1008, (1986).
Bebbington Christopher Robert
Yarranton Geoffrey Thomas
Celltech Therapeutics Limited
Vogel Nancy T.
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