Recombinant DNA inserted with hepatitis B virus gene, mammalian

Details Recombinant DNA inserted with hepatitis B virus gene, mammalian Recombinant DNA inserted with hepatitis B virus gene, mammalian

1986-07-08

1991-06-18

Weimar, Elizabeth C.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

4351723, 435 691, 435 693, 4353201, 4352402, 935 1, 935 6, 935 10, 935 12, 935 32, 935 34, 935 60, C12P 2100

Patent

active

050249380

ABSTRACT:
A recombinant DNA comprising 3 fragments of Hepatitis B virus DNA recombined with a vector consisting essentially of 0.31 Kb replication orgin of SV40 DNA inserted into EcoRI cleavage site of Escherichia coli plasmid which is deficient in the 1.426-2.521 Kb region of inhibiting replication in mammalian cells, wherein each HBV DNA fragment is a 3.2 Kb BamHI fragment consisting of 1.9 Kb HBc gene and 1.3 Kb HBs gene, and said HBV DNA fragments are arranged in a head-to-tail tandem relationship wherein the HBc gene positions at the head and the HBs gene positions at the tail, mammalian cells transformed with the recombinant DNA, and a method of production of Hepatitis B virus proteins, i.e. HBsAg and/or HBeAg. These HBV proteins have the same immunological properties as those of the natural HBV proteins originated from human blood plasma and can be used for the preparation of HBV vaccine and diagnostic reagents.

REFERENCES:
DuBois et al., PNAS USA, vol. 77, pp. 4549-4553, Aug. 1980.
Wain-Hobson et al., Chem. Abst. vol. 96:194427v, Jun. 7, 1982.
Lusky et al., Nature vol. 293, pp. 79-81, Sep. 3, 1981.
Wain-Hobson et al., Develop, Biol. Standard., vol. 50, pp. 293-300 (1982).
Liu et al., Eukanyotic Viral Vectons edited by Gluzman, CSH, pp. 55-60, J 1982.
Southern et al., Eukanyotic Viral Vectons edited by Gluzman CSH, pp. 41-45, Jun. 1982.
Breitman et al., Mol. Cell. Biol., vol. 2, pp. 966-976, Aug. 1982.
Mulligan et al., Mol. Cell. Biol., vol. 1, pp. 449-459, May 1981.

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