Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Enzymatic production of a protein or polypeptide
Patent
1984-09-26
1988-11-08
Wiseman, Thomas G.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Enzymatic production of a protein or polypeptide
4351723, 435253, 435320, 935 11, 935 29, 935 73, 935 74, 536 27, C12P 2100, C12N 1500, C12N 120, C12N 100, C07H 2100
Patent
active
047834056
ABSTRACT:
Recombinant DNA expression vectors for use in Bacillus and other host cells are disclosed. The vectors comprise the veg promoter sequence of Bacillus subtilis, a novel ribosome binding site-containing sequence and a sequence that codes for a functional polypeptide. The ribosome binding site-containing sequence is synthesized in accordance with conventional procedures while the veg promoter sequence can be obtained from E. coli K12 JA221/pMS480 (NRRL B-15258). Various sequences that codes for functional polypeptides and method for their expression in Bacillus are also disclosed.
REFERENCES:
patent: 4559300 (1985-12-01), Kovacevic et al.
Murray C. et al., in Molecular Cloning and Gene Regulation in Bacilli, Academic Press, Inc., pp. 271-285, 1982.
Matteucci, M. et al., Nuc. Acids Res., vol. 11, pp. 3113-3121, 1983.
Yansura, D. et al., in Genetics and Biotechnology of Bacilli, Academic Press, Inc., pp. 249-263, 1984.
Moran, C. et al., Mol. Gen. Genetics, vol. 186, pp. 336-346, 1982.
Palva, I. et al., Proc. Natl. Acad. Sci., vol. 79, pp. 5582-5586, 1982.
Palva, I. et al., Gene, vol. 19, pp. 81-87, 1982.
Kovacevic, S. et al., J. Bacteriol, vol. 162, pp. 521-528, 1985.
Shortle, D., Gene, vol. 22, pp. 181-189, 1983.
Hsiung Hansen M.
Kovacevic Steven
Miller James R.
Dahling Gerald V.
Eli Lilly and Company
Huleatt Jayme A.
Whitaker Leroy
Wiseman Thomas G.
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