Recombinant DNA expression vectors and DNA compounds that encode

Details Recombinant DNA expression vectors and DNA compounds that encode Recombinant DNA expression vectors and DNA compounds that encode

1987-11-02

1990-08-21

Mays, Thomas D.

Chemistry: molecular biology and microbiology

Virus or bacteriophage, except for viral vector or...

4351723, 435183, 43525233, 43525235, 435320, 536 27, 935 14, 935 29, 935 31, 935 48, 935 73, 935 75, C12N 700, C12N 1500, C12N 120, C07H 1512

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049506030

ABSTRACT:
DNA compounds encoding the Streptomyces lipmanii isopenicillin N synthetase (IPNS) gene are useful for constructing a variety of recombinant DNA vectors. The vectors are useful in producing IPNS in a wide variety of host cells, such as Streptomyces, Penicillium, and Cephalosporium. DNA compounds encoding the transcription and translation activating sequence and transcription termination sequence of the S. lipmanii IPNS gene are also useful in the construction of expression vectors, especially Streptomyces expression vectors. The S. lipmanii IPNS gene can be isolated from plasmid pOGO239, available from the Northern Regional Research Center under accession number NRRL B-18250.

REFERENCES:
patent: 4885251 (1989-12-01), Ingolia et al.
patent: 4885252 (1989-12-01), Ingolia et al.
patent: 4892819 (1990-01-01), Carr et al.
Jenson et al. Manuscript Made Available to Applicant, Oct. 1987.
Ramon et al. Gene 57:171-181, 1987.
Adelman et al., DNA 2:183-193 (1983).
Maniatis et al., Molecular Cloning 140 (1982).
Ingolia and Queener, Med. Res. Rev. 9:245-264 (1989).

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