Chemistry: molecular biology and microbiology – Vector – per se
Patent
1994-09-02
1997-01-07
Ziska, Suzanne E.
Chemistry: molecular biology and microbiology
Vector, per se
4351723, 536 241, 536 242, C12N 1500, C07H 2104
Patent
active
055916392
ABSTRACT:
The invention provides expression vectors containing the promoter, enhancer and substantially complete 5'-untranslated region including the first intron of the major immediate early gene of human cytomegalovirus. Further vectors including the hCMV-MIE DNA linked directly to the coding sequence of a heterologous gene are described, Host cells transfected with the vectors and a process for producing heterologous polypeptides using the vectors and the use of the hCMV-MIE DNA for expression of a heterologous gene are also included within the invention.
REFERENCES:
patent: 4769326 (1988-09-01), Rutter
Boshart et al in Sequence Specificity in Transcript & Translation pp. 511-520 The Strong Enhance-Element . . . 1985.
Foecking et al Gene 45:101-105. 1986.
Gruss et al PNAS 76(9):4317-4321. 1979.
Hamer et al Cell 17:725. 1979.
Mulligan et al Nature 277:108. 1979.
Spaete et al J of Virl 56(1):135, 1985.
Nelsen et al Mol. Cell Biol 7(11):4125, 1987.
Slenkey et al J of Virl 49(1):190, 1984.
Gene, vol. 38, No. 1/3.1985, Elsevier Biomedical Press, (Amsterdam, NL) F. Pasleau et al.:"Growth hormone gene expression in eukaryotic cells directed by the Rous sarcoma virus long terminal repeat or cytomegalovirus immediate--early promoter", pp. 227-232;see p. 228, second col.
Journal of Virology, vol. 56, No. 1, Oct. 1985, American Society for Microbiology (US) R. R. Spaete et al.:"Regulation of cytomegalovirus gene expression:.alpha. and .beta. promoters are trans activated by viral functions in permissive human fibroblasts" pp. 135-143, see p. 136, last five lines and first paragraph of p. 137 (cited in the application).
Wong et al., "Human GM-CSF: Molecular Cloning of the Complementary DNA and Purification of the Natural and Recombinant Proteins", Science, v. 228, pp. 810-15, May 1985.
Simonsen et al., "Isolation and expression of an altered mouse dihydrofolate reductase cDNA", Proc. Natl. Acad. Sci. (USA), vol. 80, pp. 2495-2499, 1983.
Kaufman et al., "Amplification and Expression of Sequences Cotransfected with a Modular Dihydrofolate Reductase Complementary DNA Gene", J. Mol. Biol., v. 159 pp. 601-621, 1982.
Gething et al., "Cell-surface expression of influenza haemagglutinin from a cloned DNA copy of the RNA gene", Nature, vol. 293, pp. 620-625, 1981.
McLean et al., "Cloning and expression of human lecithin-cholesterol acyltransferase cDNA", Proc. Natl. Acad. Sci. (USA), vol. 83, pp. 2335-2339, 1986.
Leonard et al., "Molecular cloning and expression of cDNAs for the human interleukin-2 receptor", Nature, vol. 311, pp. 626-631, 1984.
Gray et al., "Expression of human immune interferon cDNA in E. coli and monkey cells", Nature, vol. 295, pp. 503-508, Feb., 1982.
Nunberg et al., "Live, Recombinant Vaccinia Virus Encoding Expression of the Feline Leukemia Virus Envelope Protein", Transcriptional Control Mechanisms, (Abstract 0216), p. 154.
Treisman et al., "Transformation of rat cells by an altered polyoma virus genome expressing only the middle-T protein", Nature, vol. 292, pp. 595-600, 1981.
Celltech Ltd
Ziska Suzanne E.
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