Recombinant DNA encoding neuronal .alpha.-bungarotoxin-binding p

Details Recombinant DNA encoding neuronal .alpha.-bungarotoxin-binding p Recombinant DNA encoding neuronal .alpha.-bungarotoxin-binding p

1989-09-28

1997-02-04

Ulm, John

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

4353201, 435 691, 536 235, C12N 121, C12N 510, C12N 1512

Patent

active

055997090

ABSTRACT:
An isolated DNA molecule encoding an .alpha.-bungarotoxin-binding protein (.alpha.BgtBP) subtype or a fragment of such .alpha. subunit is provided. The fragment of the .alpha. subunit is sufficiently homologous to specified DNA (FIG. 2A or FIG. 2B) so as to hybridize thereto under conditions of low stringency. Identification, characterization, isolation and sequencing of cDNA clones which encode two neuronal .alpha.BgtBP subunits, .alpha.1 and .alpha.2, is made possible. Such clones may be used as probes to identify further neuronal .alpha.BgtBP subunits, or as diagnostic tools to screen cholinergic agents and other drugs that may affect ligand binding, ion channel or other activity of intact neuronal .alpha.BgtBP subtypes.

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