Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor
Patent
1993-01-15
1994-04-12
Wax, Robert A.
Chemistry: molecular biology and microbiology
Micro-organism, per se ; compositions thereof; proces of...
Bacteria or actinomycetales; media therefor
536 231, 536 234, 435 697, 4352523, 4353201, 530300, 530324, 530412, 530418, 935 47, 935 61, 935 72, C12N 1562, C12N 1511, C12N 1574, C07K 324
Patent
active
053025261
ABSTRACT:
Disclosed is a novel polypeptide useful as a leader or trailer peptide moiety in recombinant DNA protein production techniques involving fused protein methodology. The polypeptide comprises an amphiphilic helix designed at the DNA level to have hydrophilic charged amino acid residues on one side of the barrel of the helix and nonpolar amino acid residues on the other side of the barrel of the helix. When DNA encoding the helix is attached to a gene encoding a protein of interest, high level expression is achieved and inclusion bodies are spontaneously formed. The inclusion bodies may be collected and purified easily by altering the ionic strength and/or pH of media used to dissolve the inclusion bodies. After purification, the fused protein is cleaved to separate the amphiphilic helix from the product.
REFERENCES:
patent: 4366246 (1982-12-01), Riggs
patent: 4425437 (1984-01-01), Riggs
patent: 4431739 (1984-02-01), Riggs
patent: 4543329 (1985-09-01), Daum et al.
patent: 4563424 (1986-01-01), Riggs
patent: 4743679 (1988-05-01), Cohen et al.
patent: 4751180 (1988-06-01), Cousens et al.
patent: 5004686 (1991-04-01), Cohen et al.
patent: 5013653 (1991-05-01), Huston et al.
patent: 5215896 (1992-03-01), Keck et al.
DeGrado et al., J. American Chemical Soc., vol. 103, No. 3, 1981, pp. 679-681.
Ho et al., J. American Chemical Soc., vol. 109, No., 22, 1987 pp. 6751-6758.
Lau et al., J. Biological Chemistry vol. 259, No. 21, pp. 13253-13261; 1984.
Taylor et al., J. of Biological Chemistry, vol. 258, pp. 4464-4471, 1983.
Fukushima et al., J. Am. Chem. Soc. 101:3703 (1979).
Moe et al., Amer. Chem. Soc. 24:1972 (1985).
Musso et al., Biochem. Biophys. Res. Comm. 119:713 (1984).
Yokoyama et al., J. Biol. Chem. 225:7333 (1980).
Giniger E. et al., Nature, 330:670-672, Dec. 1987.
Marston, F. A., Review Article, Biochem. J. 240:1-2, 1986.
Kaiser, E. T., et al., Amphiphilic Secondary Structure: Design of Peptide Hormones, Science, 223, pp. 249-255, 1987.
Chou, P. Y. et al., "Empirical Predictions of Protein Conformation", Ann. Rev. Biochem., 47:251-76, 1978.
Sung, W. L., et a., Short Synthetic Oligodeoxyribonucleotide Leader Sequences Enhance Accumulation of Human Proinsulin Synthesized in Escherichia coli, PNAS, USA, 83:561-565, 1986.
Lau, S. H. et al., Surface Properties of an Amphiphlic Peptide Hormone and of its Analog: Corticotropin-releasing Factor and Sauvagine, PNAS, USA, 80: pp. 7070-7074, Dec. 1983.
Brewer and Sassenfeld, "The purification of recombinant proteins using C-terminal polyargineni fusions." Trends in Biotech. (1985) 3:119-122.
Sassenfeld and Brewer, "A polypeptide fusion designed for the purification of recombinant proteins", Biotech. (1984) 76-81.
Epand, (1983) The amphipatic helix: its possible role in the interaction of glucagen and other peptide hormones with membrane receptor sites. Trends in Biochem. Sci 8:67-69.
Blanc et al., (1983) Examination of the requirements for an amphiphilic hilical structure in .beta.-endorphin through the design, synthesis and study of model peptides. J. Biol. Chem. 258:8277-8284.
Taylor et al., (1983) Characterization of an amphiphilic helical structure in .beta.-endorphin through the design, synthesis and study of model peptides, J. Biol. Chem. 258:4464-4471.
Germino, J, et al., 1983, Proceedings of the National Academy of Sciences, U.S.A., 80(22):6848-6852.
Shoemaker, K. R., et al., 1985, Proceedings of the National Academy of Sciences, U.S.A., 82(8): 2349-2353.
Germino, J., et al., 1984, Proceedings of the National Acamedy of Sciences, U.S.A., 81(15):4692-4696.
Uhlen, M., et al., 1983, Gene, 23(3):369-378.
Nagai, K., et al., 1984, Nature, 309:810-812.
Murakami, Y., et al., 1984, Journal of the American Chemical Society, 106(12):3613--3623.
Duerden, M., et al., 1978, Journal of Theoretical Biology, 75(1):1-19.
Nagai, K., and Thogerson, H. C., 1985, Abstract of EP0161937, Derwent World Patent Index Acc. No. 85-291163/47.
Cohen Charles M.
Huston James S.
Keck Peter C.
Ridge Richard J.
Creative BioMolecules, Inc.
Moore William W.
Wax Robert A.
LandOfFree
Recombinant DNA enclosed amphiphilic leader sequences for the pr does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Recombinant DNA enclosed amphiphilic leader sequences for the pr, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Recombinant DNA enclosed amphiphilic leader sequences for the pr will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2098195