Recombinant DNA constructs and methods for controlling gene...

Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide encodes an inhibitory rna molecule

Reexamination Certificate

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C800S285000, C800S278000

Reexamination Certificate

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07816581

ABSTRACT:
The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.

REFERENCES:
patent: 2003/0018993 (2003-01-01), Gutterson et al.
patent: WO02/061101 (2002-08-01), None
U.S. Appl. No. 11/545,082, Huang, Shihshieh.
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Proudfoot (2004) “New perspectives on connecting messenger RNA 3• end formation to transcription.” Curr. Opin. Cell Biol., 16:272-278.

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