Recombinant DNA coding for signal peptide, selective interacting

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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435 693, 435 691, 435 698, 435 71, 4351721, 4352523, 4352534, 435882, 435883, 4241921, 536 235, C12N 1500

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059587369

ABSTRACT:
Tripartite recombinant DNA encoding fusion proteins which comprise three sequences, i.e., a signal peptide which is operable in a Gram positive bacterium, an immunogenic polypeptide linked thereto which is not normally expressed in a Gram positive bacterium, and a cell wall spanning and a membrane anchoring sequence, as well as their use in Gram positive bacteria to express the resultant fusion protein on their surface are described. The preferred cell wall spanning and anchoring polypeptides include Staphylococcus protein A and Streptococcus protein G.

REFERENCES:
patent: 5200327 (1993-04-01), Garvin et al.
Uhlen et al., Methods in Enzymology 185: 129-143, 1990.
Uhlen et al., Journal of Bacteriology 159(2):713-719, Aug. 1984.
Smith, Science 228: 1315-1317, Jun. 1985.

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