Recombinant DNA cloning vectors containing selectable genetic ma

Details Recombinant DNA cloning vectors containing selectable genetic ma Recombinant DNA cloning vectors containing selectable genetic ma

1984-08-10

1987-10-06

Martinell, James

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

435 68, 435 91, 4351723, 435243, 435317, 435886, 435889, 435896, 536 27, 935 6, 935 29, 935 75, 935320, C12N 120, C12N 1500, C12P 2100, C12P 1934, C12N 100, C12R 1465, C12R 1485, C12R 154, C07H 2104

Patent

active

046983074

ABSTRACT:
The present invention disclosed novel recombinant DNA cloning vectors including pMND1000 and vectors derived therefrom for use in Streptomyces and related organisms. These novel cloning vectors contain genetic markers that provide antibiotic resistance or colorimetric selectivity to the host cells. The invention further comprises transformants of the aforementioned vectors.

REFERENCES:
patent: 4416994 (1983-11-01), Nakatsukasa et al.
Rodgers et al, "Cloning and Expression of a Streptomyces fradie Neomycin Resistance Gene in Escherichia coli" Gene 18: 133 (1982).
Thompson et al, "Physical Analysis of Antibiotic-Resistance Genes from Streptomyces and Their Use in Vector Construction", Gene 20: 51 (1982).
Schupp et al, "Expression of a Neomycin Phosphotransferase Gene from Streptomyces fradiae in Escherichia coli after Interplasmidic Recombination", Mol Gen. Genet. (189: 27 (1983).
Thompson, C. et al., 1980, Nature 286:525.
Hopwood, D. A. et al., 1983, Trends in Biotechnology, 1:42.
Katz, E. et al., 1983, J. Gen. Microbiol. 129:2703.
Richardson, M. et al., 1982, Gene 20:451.

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