Recombinant DNA clone containing a genomic fragment of PfHRP-II

Details Recombinant DNA clone containing a genomic fragment of PfHRP-II Recombinant DNA clone containing a genomic fragment of PfHRP-II

1993-12-06

1995-12-19

Cunningham, Thomas M.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

435 693, 43525233, 4353201, 530350, 530395, 536 234, 536 235, C12N 121, C12N 1530, C12N 1570, C07K 14445

Patent

active

054767857

ABSTRACT:
The invention relates to isolated clones of DNA from Plasmodium falciparum that encode a histidine-rich protein from that organism. The PfHRPII protein is expressed in P. falciparum-infected erythrocytes. The cloned gene segment includes an intron-exon boundary near the amino-terminus of the coding sequence. The PfHRPII protein has a Mr of 60-80 kDa as determined by SDS-PAGE. This is substantially higher than the molecular weight of about 35 kDa as estimated from the predicted amino acid sequence of PfHRPII. The PfHRPII amino acid sequence includes a hydrophobic leader sequence, consistent with secretion of PfHRPII observed in vivo and in vivo. The amino acid sequence of PfHRPII is also characterized by a number of tandem repeats having a high content of histidine, alanine and aspartic acid.

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