Recombinant DNA, bacterium of the genus Pseudomonas containing i

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase

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435 691, 4351722, 43525234, 4353201, 536 232, 536 237, 935 9, 935 14, 935 27, 935 49, 935 72, C12N 1531, C12N 1555, C12N 1578, C12N 1567

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052906949

ABSTRACT:
A recombinant DNA capable of being replicated in a bacterium of the genus Pseudomonas is disclosed. The DNA contains a wide host range plasmid vector having a gene that codes for lipase. A process for producing lipase by transforming a host bacterium with the recombinant DNA is also described.

REFERENCES:
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Lonon, M. K., et al., 1987, Abstracts of the Annual Meeting of the American Society for Microbiology, Mar. 1-6, 1987, #K-135, p. 225.
Tsygankov, T. D. and A. Y. Chistoserdov, 1985, Plasmid 14: 118-125.
Liebl, W. and F. Gotz, 1986, Molecular and General Genetics 204:166-173.
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Joergensen, S., et al., 1991, Journal of Bacteriology 173(2): 559-567.
Journal of General Microbiology, vol. 134, 1988, pp. 433-440; S. Wohlfarth et al., "Chromosomal Mapping and Cloning of the Lipase Gene of Pseudomonas aeruginosa".
Curr. Top. Microbiol. Immunol, vol. 96, 1982, pp. 47-67; M. Bagdasarian et al., "Host Vector Systems for Gene Cloning in Pseudomonas".
Ihara, F., et al., 1992, Journal of Fermentation and Bioengineering, 73(5): 337-342.
Iizumi, T., et al., 1991, Agricultural and Biological Chemistry, 55(a): 2349-2357.
Gilman, J. P., 1953, Journal of Bacteriology, 43(2):48-52.
Buchanan, R. E. and Gibbons, N. E. Editors, Bergey's Manual of Determinative Microbiology, Eighth Edition, 1974, p. 357.

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