Recombinant DNA and transformant containing the same

Details Recombinant DNA and transformant containing the same Recombinant DNA and transformant containing the same

1997-03-31

1998-03-24

Guzo, David

Chemistry: molecular biology and microbiology

Vector, per se

435 691, 435 6951, 435 696, 435 703, 435 705, 4351721, 536 235, 536 2352, 536 241, C12N 1585, C12N 1567, C12P 2102, C07H 2104

Patent

active

057311935

ABSTRACT:
Insertion of IFN-alpha promoters in recombinant DNAs improves their expression efficiencies for useful polypeptides. Expression of such a recombinant DNA in host cells of mammalian origin is artificially controllable by the presence and absence of external stimuli using IFN-alpha inducers. Thus, transformants with such a recombinant DNA readily increase to a maximized cell density with causing neither damages nor extinction due to polypeptides they produce, and subsequent exposure to IFN-alpha inducers allows the proliferated cells to efficiently produce polypeptides with significant glycosylations.

REFERENCES:
patent: 4377513 (1983-03-01), Sugimoto et al.
patent: 4703008 (1987-10-01), Lin
patent: 4716112 (1987-12-01), Panayotatos
patent: 4808523 (1989-02-01), Revel et al.
patent: 4894334 (1990-01-01), Ben-Bassat et al.
patent: 4921699 (1990-05-01), De Chiara et al.
patent: 4939088 (1990-07-01), Young et al.
patent: 5089397 (1992-02-01), Kushner et al.
patent: 5096705 (1992-03-01), Goeddel et al.
patent: 5362490 (1994-11-01), Kurimoto et al.
Weidle, U. et al., "The 5' flanking region of a human INF-alpha gene mediates viral induction of transcription", Nature, vol. 303, No. 2, Jun. 1983, pp. 442-446.
Henco, K. et al., "Structural relationship of human interferon alpha genes and pseudogenes", Journal of Molecular Biology, vol. 185, 1985, pp. 227-260.
Recny, M. A. et al., "Structural characterization of natural human urinary and recombinant DNA-derived erythroprotein", The Journal of Biological Chemistry, vol. 262, No. 35, Dec. 1987, pp. 17156-17163.
Tanaka et al., "Cytokine gene regulation: regulatory cis-elements and DNA-binding factors involved in the interferon system", Advances in Immunology, vol. 52, 1992, pp. 263-281.
Ryals et al., "A 46-nucleotide promoter segment from an IFN-alpha gene renders an unrelated promoter inducible by virus", Cell, vol. 41, Jun. 1985, pp. 497-507.
Abstract, Database WPI, Section Ch, Week 8617, Derwent Publications Ltd., London, GB; Class B04, AN 86-109964, XP002016726 & JP-A-61 052 286 (Toray Ind Inc), Mar. 14, 1986.
Mori et al., "A high-level and regulatable production system for recombinant glycoproteins using a human interferon-alpha promoter-based expression vector", Gene, vol. 144, No. 2, Jul. 8, 1994.
Craig, E.A. et al.; "Sequence Organization of Two Recombinant Plasmids Containing Genes for the Major Heat Shock-Induced Protein of D. melanogaster"; Cell, vol. 16, pp. 575-599 (1979).
Brinster, R.L. et al.; "Somatic Expresion of Herpes Thymidine Kinase In Mice Following Injection Of A Fusion Gene Into Eggs"; Cell, vol. 27, pp. 223-231, (1981).
Lee, F. et al.; Glucocorticoids Regulate expression Of Dihydrofolate Reductase cDNA In Mouse Mammary Tumour Virus CHimaeric Plasmids; Nature, vol. 294, pp. 228-232, (1981).
Fukunaga et al. "Constitutive Production of Human Interferons by Mouse Cells . . . " PNAS 81 5086-5090 1984.
Mori et al. "A High Level & Regulatable Production System for Recombinant Glycoproteins . . . " Gene 144 289-293 1994.
Dron et al. "Priming Affects the Activity of a Specific Region of the Promoter of the Human Beta Interferon Gene" Mol Cell Biol 10(2) 854-858 1990.

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