Recombinant DNA and expression vector for the repressible and in

Details Recombinant DNA and expression vector for the repressible and in Recombinant DNA and expression vector for the repressible and in

1995-02-09

1998-11-03

Degen, Nancy

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

235 691, 2352523, 23525333, 23525234, 536 231, 536 241, C12N 1563, C12N 121, C07H 2104, C12P 2102

Patent

active

058307206

ABSTRACT:
The recombinant DNA according to the present invention contains a regulator region which is different from the pfl gene; a promoter region upstream from the gene which contains a -35/-10 promoter sequence; a regulator region upstream from the promoter region which contains a sequence (I) 5'-GAGATATGATCTATATCAATTTC-3' or a 23 base pair sequence which is at least 80% identical to positions 6-10 and 15-19 of said sequence (I) but not having C at position 8 and/or G at position 17. An expression vector according to the present invention contains a recombinant DNA according to the present invention ligated into a suitable vector molecule and methods are also described for expression the DNA.

REFERENCES:
patent: 4628027 (1986-12-01), Gay
patent: 4767843 (1988-08-01), Yazaki et al.
patent: 5071963 (1991-12-01), Revel et al.
Maniatis et al. "Molecular Cloning: A Laboratory Manual" (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.) pp. 403-433 1982.
Jayaraman et al., Molecular Microbiology, "The nirB promotor of Escherichia coli: location of nucleotide sequences essential for regulation by oxygen, the FNR protein and nitrite", (1988) 2(4), pp. 527-530.
Li et al., Journal of Bacteriology, "Promotor Region of the nar Operon of Escherichia coli Nucleotide Sequence and Transcription Initiation Signals", Oct. vol. 169, No. 10, Oct. 1987, pp. 4614-1620.
Sawers et al., "Anaerobic Regulation of Pyruvate Formate-Lyase from Escherichia coli K-12", Journal of Bacteriology, Band 170, Nov. 11, 1988, pp. 5330-5336.
Sawers et al., "Novel Transcriptional Control of the Pyruvate Formate-Lyase Gene: Upstream Regulatory Sequences and Multiple Promoters Regulate Anaerobic Expression", Journal of Bacteriology, Band 171, May 5, 1989, pp. 2485-2498.
Biological Abstract v. 75(11), 1983, Abstract No. 80078.
Biochemistry, 1983, vol. 22, pp. 5858-5868, Robert McCarrol et al, "Nucleotide Sequence of the Dictyostelium discoideum Small-Subunit Ribosomal Ribonucletic Acid Inferred from the Gene Sequence: Evolutionary Implications".
Journal of Bacteriology, May 1989, vol. 171, No. 5 pp. 2485-2498, by Sawyers and Bock, "Novel Transcriptional Control of the Pyruvate Formate-Lyase Gene: Upstream Regulatory Sequence and Multiple Promoters Regulate Anaerobic Expression".
Archives of Microbiology, 1982, vol. 132, pp. 365-371, by Pecher et al., "Expression of Pyruvate Formate-Lyase of Escherichia coli from the Cloned Structural Gene".

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