Recombinant DNA and a process for producing phosphotransacetylas

Details Recombinant DNA and a process for producing phosphotransacetylas Recombinant DNA and a process for producing phosphotransacetylas

1989-12-19

1992-12-29

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Transferase other than ribonuclease

4351723, 43525233, 4353201, C12N 912, C12N 1570, C12N 1572

Patent

active

051751046

ABSTRACT:
A novel recombinant DNA is characterized in that DNA containing a gene encoding phosphotransacetylase has been inserted into vector DNA. The recombinant DNA can be obtained by culturing in medium E. coli 1100 (pPT200) (FERM BP-2195) belonging to the genus Escherichia which contains a recombinant DNA obtained by inserting DNA containing a gene coding for phosphotransacetylase into vector DNA and is capable of producing phosphotransacetylase, and collecting phosphotransacetylase from the culture.

REFERENCES:
Jacobs, et al., Nature 313:806-810, (1985).
Itaya, et al., (1967), Agr. Biol. Chem., 31:1256-1264 Van Holde, Physical Biochemistry, Prentice-Hall, 1971, p. 108.
Biochem. Biophys. Acta, 191, 550-558 (1969), Entitled: "Phosphotransacetylase of Escherichia Coli B, Purification and Properties" by Masao Shimizu, Tadao Suzuki, Kin--Ya-Kameda and Yasushi Abiko.

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