Recombinant .DELTA.9 desaturase and a gene encoding the same

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase

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4352523, 4353201, 536 232, C12N 902, C12N 121, C12N 1563, C07H 2104

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active

056396457

ABSTRACT:
An isolated gene encoding .DELTA.9 desaturase of cyanobacteria, an expression vector containing the same, a transformant transformed therewith and a recombinant .DELTA.9 desaturase are provided, wherein said gene is useful for improving the composition of fatty acids of animals, plants and microorganisms by transformation and for producing animals, plants or microorganisms which tolerate low temperature.

REFERENCES:
patent: 5552306 (1996-09-01), Thomas et al.
Higashi, S. et al. (1993) "An in vivo study of substrate specificities of acyl-lipid desaturases and acyltransferases in lipid synthesis in Synechocystis PCC6803" Plant Physiol. 102(4):1275-1278. Aug. 1993.
Wada et al. Nature vol. 347 13 Sep. 1990, pp. 200-203.
Reddy et al. Plant Molecular Biology 27: pp. 293-300, 1993.
Sakamoto et al. Plant Molecular Biology 24: pp. 643-650, 1994.
H. Wada et al., "Temperature-Induced Changes in the Fatty Acid Composition of the Cyanobacterium, Synechocystis PCC6803.sup.1n ", Plant Physiol. vol. 92, pp. 1062-1069, 1990.
H. Wada et al., "Enhancement of Chilling Tolerance of a Cyanobacterium by Genetic Manipulation of Fatty Acid Desaturation", Letters to Nature, vol. 347, pp. 200-203, Sep. 13, 1990.
T. Sakamoto et al., ".DELTA.9 Acyl-Lipid Desaturases of Cyanobacteria", The Journal of Biological Chemistry, vol. 269, No. 41, pp. 25576-25580, Oct. 14, 1994.

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