Recombinant cytomegalovirus containing foreign gene and use ther

Chemistry: molecular biology and microbiology – Virus or bacteriophage – except for viral vector or...

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4351723, 4353201, 536 231, 536 237, 536 241, C12N 701, C12N 1500, C12N 1511, C12N 1586

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058307451

ABSTRACT:
The present invention provides a virus comprising a DNA sequence essential for replication of a human cytomegalovirus and at least one foreign DNA sequence adapted for expression in a host. The foreign DNA sequence may encode a human immunodeficiency virus anti-sense mRNA sequence or an antigenic polypeptide, e.g., a malarial surface antigen. Also provided are therapeutic compositions and vaccines which comprise the novel viruses of the present invention.

REFERENCES:
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patent: 5168062 (1992-12-01), Stinski
patent: 5273876 (1993-12-01), Hock et al.
patent: 5385839 (1995-01-01), Stinski
patent: 5561063 (1996-10-01), Hock et al.
Foecking et al. "Poweful & Versatile Enhancer-Promoter Unit for Gammalian Expression Vectors" Mene 45 1986 101-105.
Thomsen et al. "Promoter Regulatory Region of the Major Immediate Ealy Gene of Human Cytomegalovirus" PNAS 81659-663 1984.
Tamashiro et al. "Constructing a Library of the ELori Fragments . . . " J. Vhol. 42(2) 547-557 1982.

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