Recombinant cyclopentanone monooxygenase [cpmo]

Details Recombinant cyclopentanone monooxygenase [cpmo] Recombinant cyclopentanone monooxygenase [cpmo]

2007-03-28

2009-06-02

Nashed, Nashaat T (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Oxidoreductase

C536S023200

Reexamination Certificate

active

07541168

ABSTRACT:
Cyclopentanone 1,2-monooxygenase (CPMO) fromComamonas(previouslyPseudomonas) sp. strain NCIMB 9872 carries out the second step of a degradation pathway that allows the bacterium to use cyclopentanol as a sole carbon source for growth. In the present invention there is reported the localization of the CPMO-encoding gene (cpnB) on a 4.3-kb SphI fragment, the determination of its sequence. The 550-amino acid CPMO polypeptide (Mr, 62,111) encoded by the gene was found to have 36.5% identity with the sequence of cyclohexanone 1,2-monooxygenase (CHMO) ofAcinetobactersp. strain NCIMB 9871. The 62-kDa CPMO was expressed inE. colias an IPTG-inducible protein.

REFERENCES:
patent: 5017495 (1991-05-01), Yen et al.
patent: 5079166 (1992-01-01), Winter et al.
patent: 5114852 (1992-05-01), Yabusaki et al.
patent: 5171684 (1992-12-01), Yen et al.
patent: 5605823 (1997-02-01), Yen et al.
patent: 5916791 (1999-06-01), Hirschberg et al.
patent: 5928921 (1999-07-01), Hayashi et al.
patent: 5942426 (1999-08-01), Hayashi et al.
patent: 5965795 (1999-10-01), Hirschberg et al.
patent: 6100446 (2000-08-01), Streber et al.
patent: 6140512 (2000-10-01), Adger et al.
patent: 6153401 (2000-11-01), Streber et al.
patent: 6218599 (2001-04-01), Hirschberg et al.
patent: 86139 (1983-08-01), None
patent: 247600 (1987-12-01), None
patent: 725137 (1996-08-01), None
patent: 999274 (2000-05-01), None
patent: 1006191 (2000-06-01), None
patent: 62-228276 (1987-10-01), None
patent: 1-074983 (1989-03-01), None
patent: 2-119777 (1990-05-01), None
patent: 9-121864 (1997-05-01), None
patent: 10-099078 (1998-04-01), None
patent: WO 98/24914 (1998-06-01), None
patent: WO 98/38294 (1998-09-01), None
patent: WO 98/46762 (1998-10-01), None
patent: WO 00/09682 (2000-02-01), None
patent: WO 01/07629 (2001-02-01), None
patent: WO 01/07630 (2001-02-01), None
patent: WO 01/11026 (2001-02-01), None
patent: WO 01/14574 (2001-03-01), None
patent: WO 01/20011 (2001-03-01), None
patent: WO 01/31047 (2001-05-01), None
patent: WO 01/36654 (2001-05-01), None
patent: WO 01/38500 (2001-05-01), None
Bes et al. Oxidative biotransformations by microorganisms: production of chiral synthons by cyclopentanone monooxygenase from Pseudomonas sp. NCIMB 9872, J Molecular Catalysis B: Enzymatic, 1(3): 127-134, 1996.
Chen et al. (Acinetobacter cyclohexanone monooxygenase: gene cloning and sequence determination, J Bacteriol. Feb. 1988; 170(2): 781-9.
Altschul et al., 1997, Nucleic Acids Res., 25 : 3389-3402.
Birnboim and Doly, 1979, DNA Nucleic Acids Res., 7 : 1513-1523.
Bradford, 1976, Anal. Biochem., 72: 248-254.
Cha et al., 1993, Gene, 136: 369-370.
Chen et al., 1988, J. Bacteriol., 170: 781-789.
Donoghue et al., 1976, Eur. J. Biochem., 63: 175-192.
Enroth et al., 1998, Structure, 6 : 605-617.
Eppink et al., 1997, Prot. Sci., 6: 2454-2458.
Griffin et al., 1972, Biochem J., 129: 595-603.
Griffin et al., 1976, Eur. J. Biochem., 63 : 199-209.
Jornvall et al., 1995, Biochemistry, 34: 6003-6013.
Kokotek et al., 1989, Gene, 84: 467-471.
Kruger et al., 1992, J. Bacteriol., 179: 4391-4400.
Marmur, 1961, J. Mol. Biol., 3: 208-218.
Miranda et al., 1987, The complete nucleotide sequence of the gInALG operon ofEschericha coliK12, 15: 2757-2770.
Morett and Segovia, 1993, J. Bacteriol., 175: 6067-6074.
Morii et al., 1998, GenBank accession No. AB010439.
Sambrook et al., 1989, Molecular cloning: a laboratory manual, 2nded., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
Smith et al., 1996, Biochemistry, 35: 8805-8814.
Stewart, 1998, Curr. Org. Chem., 2: 195-216.
Stewart et al., 1998, J. Am. Chem. Soc., 120 : 3541-3548.
Stinson, 1998, Chem. Eng. News., 83-104.
Willetts, 1997, Trends in Biotech., 15: 55-62.
Yanisch-Perron et al., 1985, Gene, 33: 103-119.

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