Recombinant cyclodextran glucanotransferase mutants

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Transferase other than ribonuclease

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435 97, C12N 910, C12P 1918

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active

058044264

ABSTRACT:
A recombinant cyclodextrin glucanotransferase mutant is provided, which mutant produces lowered amounts of .beta.-cyclodextrins and .gamma.-cyclodextrins compared to wild-type cyclodextrin glucanotransferase.

REFERENCES:
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Fujiwara et al. (1992) Cyclization Characteristics of Cyclodextrin Glucanotransferase Are Conferred by the NH2-Terminal Region of the Enzyme, Applied and Environmental Microbiology, 58 (12): 4016-4025, Dec. 1992.
Yamane et al. (1993) Cyclomaltodextrin, glucanotransferase (CGtase) muteins for enhanced preparation of gamma-cyclodextrin, CAPLUS, CAPLUS ACCES, No. 1993:423687 CAPLUS (119:23687), Feb. 23, 1993.
Yamane et al. (1990) Cyclomaltodextrin glucanotransferase derivatives of Bacillus for improved yield of alpha-cyclodextrin, CAPLUS, CAPLUS ACCESS. No. 1990:153084 CAPLUS (112:153084), Sep. 12, 1988.
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